Protective effects of chrysin against the neurotoxicity induced by aluminium: In vitro and in vivo studies
| Autor: | Hericles Mesquita Campos, Lorrane Kelle da Silva Moreira, Andrea Tarozzi, Letizia Pruccoli, Elson Alves Costa, Paulo César Ghedini, Fernanda C. A. Santos, Hiasmin Franciely da Silva Neri, Cinthia Rio Branco da Silva, Michael da Costa |
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| Přispěvatelé: | Campos H.M., da Costa M., da Silva Moreira L.K., da Silva Neri H.F., Branco da Silva C.R., Pruccoli L., dos Santos F.C.A., Costa E.A., Tarozzi A., Ghedini P.C. |
| Rok vydání: | 2022 |
| Předmět: |
Male
Antioxidant THP-1 Cells medicine.medical_treatment Anti-Inflammatory Agents Pharmacology GPI-Linked Proteins Toxicology medicine.disease_cause Neuroprotection Antioxidants Protein Carbonylation Lipid peroxidation Superoxide dismutase Mice Necrosis chemistry.chemical_compound In vivo Cell Line Tumor Aluminium Neurotoxicity medicine Aluminum Chloride Animals Humans Chrysin Flavonoids Neurons Behavior Animal biology Brain medicine.disease Disease Models Animal Neuroprotective Agents chemistry Oxidative stress Butyrylcholinesterase Acetylcholinesterase Exploratory Behavior biology.protein Neurotoxicity Syndromes Lipid Peroxidation Inflammation Mediators Locomotion |
| Zdroj: | Toxicology. 465:153033 |
| ISSN: | 0300-483X |
| Popis: | Chronic exposure to aluminium (Al) can contribute to the progression of several neurological and neurodegenerative diseases. Al is a metal that promotes oxidative damage leading to neuronal death in different brain regions with behavior, cognition, and memory deficits. Chrysin is a flavonoid found mainly in honey, passion fruit, and propolis with antioxidant, anti-inflammatory, and cytoprotective properties. In this study, we used an integrated approach of in vitro and in vivo studies to evaluate the antioxidant and neuroprotective effects of chrysin against the neurotoxicity elicited by aluminium chloride (AlCl3). In in vitro studies, chrysin (5 μM) showed the ability to counteract the early oxidative stress elicited by tert-butyl hydroperoxide, an oxidant that mimics the lipid peroxidation and Fenton reaction in presence of AlCl3 as well as the late necrotic death triggered by AlCl3 in neuronal SH-SY5Y cells. In vivo studies in a mouse model of neurotoxicity induced by chronic exposure to AlCl3 (100 mg/kg/day) for ninety days then corroborated the antioxidant and neuroprotective effect of chrysin (10, 30, and 100 mg/kg/day) using the oral route. In particular, chrysin reduced the cognitive impairment induced by AlCl3 as well as normalized the acetylcholinesterase and butyrylcholinesterase activities in the hippocampus. In parallel, chrysin counteracted the oxidative damage, in terms of lipid peroxidation, protein carbonylation, catalase, and superoxide dismutase impairment, in the brain cortex and hippocampus. Lastly, necrotic cells frequency in the same brain regions was also decreased by chrysin. These results highlight the ability of chrysin to prevent the neurotoxic effects associated with chronic exposure to Al and suggest its potential use as a food supplement for brain health. |
| Databáze: | OpenAIRE |
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