The use of high-performance liquid chromatography with diode array detector for the determination of sulfide ions in human urine samples using pyrylium salts
| Autor: | A. Nowicki, Grażyna Chwatko, W. Zgagacz, Katarzyna Urbaniak, Robert Zakrzewski |
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| Přispěvatelé: | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Poland, Department of Organic and Applied Chemistry, Faculty of Chemistry, University of Lodz, Poland |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Sulfide
Calibration curve Hydrogen sulfide Clinical Biochemistry Human urine sample High performance liquid chromatography with diode detector (DAD) Sulfides 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine sole piryliowe Limit of Detection Chromatography detector Pyrylium salts Benzene Derivatives Humans Derivatization Chromatography High Pressure Liquid detektor diodowy (DAD) chemistry.chemical_classification Chromatography wysokosprawna chromatografia cieczowa (HPLC) Chemistry 010401 analytical chemistry Chlorate jon siarczkowy mocz Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences Sulfide ions Linear Models Salts Sample collection |
| Popis: | Hydrogen sulfide is a toxic gas involved in the regulation of some essential biological processes. A novel, precise, accurate and rapid method based on high-performance liquid chromatography with diode array detection for the determination of sulfide ions in human urine sample is proposed. The method involves the derivatization of sulfide with pyrylium salts – (2,4,6-triphenylpyrylium hydrogensulfate(VI) (L1) and 4-[p-(N,N-dimethylamino)phenyl]-2,6-diphenylpyrylium chlorate(VII) (LN1). The separation occurs on InfinityLab Poroshell 120 EC C18 column using acetonitrile and phosphate buffer as a mobile phase. The detectors utilized a wavelength of 371 or 580 nm. The calibration curves were linear in the range of 2–150 μmol L−1 and 1–50 μmol L−1 for L1 and LN1 derivatives, respectively. The samples were found to be stable from sample collection to final analysis. The method was successfully applied to samples from apparently healthy volunteers. |
| Databáze: | OpenAIRE |
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