The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions
| Autor: | Flávia Goulart da Rosa Cardoso, Renata Falchete do Prado, Bruna Ribas de Meireles, Cláudio Antonio Talge Carvalho, Felipe de Souza Matos, Marcia Carneiro Valera, Rayana Duarte Khoury, Luciane Dias de Oliveira |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Propranolol Bone resorption 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phentolamine Adrenergic Agents Osteoprotegerin Apical periodontitis Corticosterone Stress Physiological Internal medicine medicine Animals Chronic stress Bone Resorption Rats Wistar General Dentistry Inflammation biology Adrenergic blockers business.industry RANK Ligand Radioimmunoassay 030206 dentistry Cell Biology General Medicine X-Ray Microtomography Rats Receptors Adrenergic Oxidative Stress 030104 developmental biology Endocrinology Otorhinolaryngology chemistry RANKL biology.protein business Periapical Periodontitis medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1879-1506 |
| Popis: | Made available in DSpace on 2020-12-12T02:29:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To investigate the influence of chronic stress and adrenergic blockade in a rat model of apical periodontitis. Methods: Thirty-two Wistar rats were submitted to an animal model of periapical lesion and randomly divided into 4 groups (n = 8): no stress (NS); stress + saline solution (SS); stress + β-adrenergic blocker (Sβ); stress + α-adrenergic blocker (Sα). The SS, Sβ and Sα groups were submitted to an animal model of chronic stress for 28 days and received daily injections of saline solution, propranolol (β adrenergic blocker) and phentolamine (α adrenergic blocker), respectively. After 28 days the animals were euthanized and the following analyses were carried out: a) serum corticosterone levels through Radioimmunoassay; b) measurement of serum levels of IL-1B, IL-6, IL-10 and IL-17 by enzyme-linked immunosorbent assay (ELISA); c) volume of periapical bone resorption by micro-computed tomography; d) histomorphometric analysis by staining with hematoxylin and eosin; e) expression of β-AR, α-AR, receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) by immunohistochemistry; f) tartrate-resistant acid phosphatase (TRAP) staining; g) ex-vivo cytokine release followed by the stimulation with LPS in superfusion system, by ELISA. Results: SS group displayed significantly higher corticosterone levels than NS group (non-stressed). Higher IL-1β serum level was observed in the NS group (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect