Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
| Autor: | Paulo Vieira, Kathryn Kamo, Cláudia S. L. Vicente, Lev G. Nemchinov, Jonathan D. Eisenback, Manuel Mota |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nematoda Glycobiology Nicotiana benthamiana Plant Science 01 natural sciences Biochemistry Pratylenchus penetrans Database and Informatics Methods Medicine and Health Sciences Nematode Infections Flowering Plants Multidisciplinary biology Heteropolysaccharides Sequence analysis food and beverages Eukaryota Plants Medicine Pectins medicine.symptom Cellular Structures and Organelles Plant Cell Walls Cellular Types Research Article Nicotiana Bioinformatics Science Plant Cell Biology Research and Analysis Methods Lesion Cell wall 03 medical and health sciences Cell Walls Amino Acid Sequence Analysis Polysaccharides Plant Cells medicine Parasitic Diseases Animals Gene DNA sequence analysis fungi Organisms Biology and Life Sciences Cell Biology biology.organism_classification Molecular biology Invertebrates Pectin methyl esterase 030104 developmental biology Nematode Sequence Alignment Bacteria 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 2, p e0212540 (2019) Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| ISSN: | 1932-6203 |
| Popis: | Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda. |
| Databáze: | OpenAIRE |
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