Synovial effusion and synovial fluid biomarkers in psoriatic arthritis to assess intraarticular tumor necrosis factor-α blockade in the knee joint

Autor: Francesca Lunardi, S. Dainese, Fiorella Calabrese, Beatrice Molena, L Cozzi, Leonardo Punzi, Pascale Roux-Lombard, Leopoldo Rubaltelli, Paolo Sfriso, M. Vezzù, Francesca Oliviero, Elena Scagliori, Anna Scanu, Ugo Fiocco, Jean-Michel Dayer, R. Nardacchione
Rok vydání: 2010
Předmět:
Pathology
Knee Joint
Interleukin-1beta
Interleukin-6/metabolism
Arthritis
Injections
Intra-Articular
Leukocyte Count
Biological Markers/*metabolism
Synovial Fluid
Interleukins/metabolism
Immunology and Allergy
ddc:616
Arthritis
Psoriatic/drug therapy/metabolism/pathology
biology
Synovial Membrane
medicine.anatomical_structure
Tumor necrosis factor alpha
Drug Monitoring
Immunosuppressive Agents
Research Article
musculoskeletal diseases
medicine.medical_specialty
Tumor Necrosis Factor-alpha/*antagonists & inhibitors
Immunology
Psoriatic arthritis
Rheumatology
Immunosuppressive Agents/*administration & dosage
Internal medicine
Synovial Fluid/*metabolism
medicine
Humans
Synovial fluid
ddc:610
Interleukin 6
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Interleukins
Arthritis
Psoriatic
Reproducibility of Results
Synovial Membrane/metabolism/pathology
medicine.disease
Interleukin 1 Receptor Antagonist Protein
Knee Joint/metabolism/pathology
Drug Monitoring/*methods/standards
Interleukin-1beta/metabolism
biology.protein
Synovial membrane
business
Biomarkers
Interleukin 1 Receptor Antagonist Protein/metabolism
Zdroj: Arthritis Research and Therapy, Vol. 12, No 4 (2010) P. R148
Arthritis Research & Therapy
ISSN: 1478-6354
Popis: INTRODUCTION: The purpose of this study was the evaluation of synovial effusion (SE), synovial fluid (SF) and synovial tissue (ST) biomarkers in relation to disease activity indexes to assess the response to intraarticular (IA) tumor necrosis factor (TNF)-alpha blockers in psoriatic arthritis (PsA). METHODS: Systemic and local disease activity indexes (disease activity score (DAS); the Ritchie articular index (mRAI), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP); Thompson articular (THOMP) and joint articular (KJAI)-Index ) and ST samples were assessed at baseline, throughout treatment, and during the follow-up in 14 patients affected with PsA who underwent IA injections (0.5 ml to 12.5 mg) in the knee joint of etanercept (E) or placebo (P) once every two weeks for a 10-week period. Total SF white blood cell (WBC) counts (WBC/mul) and SF cytokine/chemokine (CK/CCK) levels were measured before IA-E at baseline, after IA-E, and as long as there were adequate amounts of SF for knee aspiration (post). Characterization of synovial mononuclear cell infiltration and synovial vessels was carried out in 8 out of 14 knees by staining serial sections of synovial tissue biopsies for CD45, CD3, CD68, CD31 and CD105. RESULTS: At baseline, CRP and/or ESR were significantly correlated with SF-CK (interleukin- (IL-)1beta, IL-1Ra, IL-6, IL-8) and CCK (CCL3). Post-IA injections, there was a decrease in SE in the knees in which aspiration following IA-E injection was possible as well as a significant reduction in SF WBC/mul and in SF-CK (IL-1beta, IL-1Ra, IL-6 and IL-22). Pre- and post-IA-E injections, there were significant correlations between ST markers and SF-CK (IL-1beta with CD45; IL-1beta and IL-6 with CD31) and between SF-CCK (CCL4 and CCL3 with CD3). At the end of the study, there was a significant reduction in disease activity indexes (CRP, DAS, RAI, THOMP, KJAI) as well as in the ST markers (CD45; CD3). CONCLUSIONS: Synovial effusion regression is a reliable indicator of the response to IA TNF-alpha blockers in PsA patients as it is confirmed by the correlation between SF biomarkers to disease activity and synovial tissue inflammation.
Databáze: OpenAIRE
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