Expressed sequence tags and molecular cloning and characterization of gene encoding pinoresinol/lariciresinol reductase from Podophyllum hexandrum
| Autor: | Dipul Kumar Biswas, Dhammaprakash Pandhari Wankhede, Alok Krishna Sinha, S. Rajkumar |
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| Rok vydání: | 2013 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Plant Science Genes Plant Lignans chemistry.chemical_compound Gene Expression Regulation Plant NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases Stress Physiological medicine Amino Acid Sequence Cloning Molecular Furans Secoisolariciresinol dehydrogenase Phylogeny Gene Library Plant Proteins Podophyllotoxin Expressed Sequence Tags Genetics Lignan Expressed sequence tag Base Sequence biology Gene Expression Profiling Podophyllum Sequence Analysis DNA Cell Biology General Medicine biology.organism_classification Biosynthetic Pathways Alcohol Oxidoreductases Dirigent protein Pinoresinol chemistry biology.protein Oxidoreductases Sequence Alignment medicine.drug |
| Zdroj: | Protoplasma. 250:1239-1249 |
| ISSN: | 1615-6102 0033-183X |
| DOI: | 10.1007/s00709-013-0505-z |
| Popis: | Podophyllotoxin, an aryltetralin lignan, is the source of important anticancer drugs etoposide, teniposide, and etopophos. Roots/rhizome of Podophyllum hexandrum form one of the most important sources of podophyllotoxin. In order to understand genes involved in podophyllotoxin biosynthesis, two suppression subtractive hybridization libraries were synthesized, one each from root/rhizome and leaves using high and low podophyllotoxin-producing plants of P. hexandrum. Sequencing of clones identified a total of 1,141 Expressed Sequence Tags (ESTs) resulting in 354 unique ESTs. Several unique ESTs showed sequence similarity to the genes involved in metabolism, stress/defense responses, and signalling pathways. A few ESTs also showed high sequence similarity with genes which were shown to be involved in podophyllotoxin biosynthesis in other plant species such as pinoresinol/lariciresinol reductase. A full length coding sequence of pinoresinol/lariciresinol reductase (PLR) has been cloned from P. hexandrum which was found to encode protein with 311 amino acids and show sequence similarity with PLR from Forsythia intermedia and Linum spp. Spatial and stress-inducible expression pattern of PhPLR and other known genes of podophyllotoxin biosynthesis, secoisolariciresinol dehydrogenase (PhSDH), and dirigent protein oxidase (PhDPO) have been studied. All the three genes showed wounding and methyl jasmonate-inducible expression pattern. The present work would form a basis for further studies to understand genomics of podophyllotoxin biosynthesis in P. hexandrum. |
| Databáze: | OpenAIRE |
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