Cloning, Expression, and Characterization of a Thermostable PAP2L2, a New Member of the Type-2 Phosphatidic Acid Phosphatase Family fromGeobacillus toebiiT-85
| Autor: | Xie Yi, Shaohua Gu, Yumin Mao, Jing Peng, Zhenxing Yang, Xiaodong Huang, Xiangwei Fei, Chaoneng Ji, Yong Zhang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Molecular Sequence Data
Phosphatase Phosphatidate Phosphatase Gene Expression Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Affinity chromatography Enzyme Stability Escherichia coli medicine Geobacillus toebii Amino Acid Sequence Cloning Molecular Bacillaceae Molecular Biology Peptide sequence chemistry.chemical_classification Base Sequence Organic Chemistry Temperature Sequence Analysis DNA General Medicine Phosphatidic acid biology.organism_classification Molecular biology Recombinant Proteins Amino acid Enzyme chemistry Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 72:3134-3141 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | Most members of the type-2 phosphatidic acid phosphatase (PAP2) superfamily are integral membrane phophatases involved in lipid-related signal transduction and metabolism. Here we describe the cloning of a novel gene from Geobacillus toebii T-85, encoding a PAP2-like protein, Gtb PAP2L2, which contains 212 amino acids and shows a limited homology to other known PAP2s, especially at conserved phosphatase motifs, and a similar six-transmembrane topology structure. This enzyme was expressed, and purified in Escherichia coli. Recombinant Gtb PAP2L2s from the membrane fractions were solublized with 0.3% (v/v) Triton X-100 and purified by Ni(2+) affinity chromatography. The purified enzyme showed broad substrate specificity to phosphatidic acid, diacylglycerol pyrophosphate, and lysophosphatidic, but preferred phosphatidic acid and diacylglycerol pyrophosphate in vitro. Gtb PAP2L2 is a thermal stable enzyme with a half-life of 30 min at 60 degrees C. The enzyme was strongly inhibited by 1% SDS, 10 mM veranda, and Zn(2+), whereas it was independent of the Mg(2+) ion, and insensitive to N-ethylmaleimide. The purified recombinant Gtb PAP2L2 was catalytically active and highly stable, making it ideal as a candidate on which to base further PAP2 structure/function studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|