Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
| Autor: | Andrei Kuzminov, Nayun Kim, Juan Lucas Argueso, Elina A. Radchenko, Sharik R. Khan, Catherine H. Freudenreich, Sang Eun Lee, Cynthia J. Sakofsky, Erica J Polleys, Mathew Stracy, Sue Jinks-Robertson, James E. Haber, Dmitry A. Gordenin, Richard D. Kolodner, Anna Malkova, Giedrė Bačinskaja, Judith Miné-Hattab, Lea Marie, Sergei M. Mirkin, Jun Che, Zhenxin Yan, Devon M. Fitzgerald, Belén Gómez-González, Lorraine S. Symington, Rajula Elango, Jun Xia, Eun Yong Shim, Andrés Aguilera, Pawel Zawadzki, Megan C. King, Anais Cheblal, Thomas D. Petes, Susan M. Gasser, Kyle M. Miller, Sarah Lambert, Michael Lisby, Susan M. Rosenberg, Anissia Ait Saada, Bryan A Leland, Anastasiya Epshtein, Sandeep Kumar, Qian Mei, Hannah L. Klein, Grzegorz Ira, Alicja Piotrowska, Yi Yin, Christopher D. Putnam, Rodney Rothstein |
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| Přispěvatelé: | Physico-Chimie-Curie (PCC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC), Stress génotoxiques et cancer, Université Paris-Sud - Paris 11 (UP11)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS), The Research Institute for Water Security, Wuhan University, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Universidad de Sevilla, Friedrich Miescher Institute for Biomedical Research (FMI), Novartis Research Foundation, Rosenstiel Basic Medical Sciences Research Center [Waltham], Brandeis University, Department of Molecular and Human Genetics [Houston, TX, USA], Baylor College of Medecine, Department of Biology [Copenhagen], Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Ministerio de Economía y Competitividad (España), European Research Council, Junta de Andalucía, Swiss National Science Foundation, National Institutes of Health (US), Novartis, National Science Foundation (US), Fondation pour la Recherche Médicale, Agence Nationale de la Recherche (France), W. M. Keck Foundation, Cancer Prevention and Research Institute of Texas, National Science Centre (Poland), Danish Natural Science Research Council, Universidad de Sevilla. Departamento de Genética, Centre National de la Recherche Scientifique (CNRS)-Institut Curie-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Paris-Sud - Paris 11 (UP11)-Institut Curie-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Virologie et Immunologie Moléculaires (VIM) |
| Rok vydání: | 2019 |
| Předmět: |
Genome instability
gene amplification [SDV]Life Sciences [q-bio] homologous recombination Review Applied Microbiology and Biotechnology Genome DNA repair centers 0302 clinical medicine crossovers Gene duplication yeast artificial chromosome lcsh:QH301-705.5 ComputingMilieux_MISCELLANEOUS 0303 health sciences gene conversion sister chromatid recombination R-loops 3. Good health site-specific chromosome breaks gross chromosome rearrangements mitotic recombination Holliday junctions sister repetitive sequences chromatin dynamics DSBs replication fork stalling mutagenesis Biotechnology Mitotic crossover DNA repair fluorescent proteins toxic recombination intermediates Mutagenesis (molecular biology technique) Computational biology pulsed field gel electrophoresis Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology 03 medical and health sciences Virology Genetics [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Gene conversion DNA breaks Molecular Biology 030304 developmental biology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology DNA resection genome instability chromosome rearrangements lcsh:Biology (General) Parasitology single-particle tracking Generic health relevance Homologous recombination 030217 neurology & neurosurgery |
| Zdroj: | Microbial cell (Graz, Austria), vol 6, iss 1 Microbial Cell, Vol 6, Iss 1, Pp 1-64 (2019) Microbial Cell Microbial Cell, Shared Science Publishers OG, 2019, 6 (1), pp.1-64. ⟨10.15698/mic2019.01.664⟩ Digital.CSIC. Repositorio Institucional del CSIC instname idUS. Depósito de Investigación de la Universidad de Sevilla Klein, H L, Bacinskaja, G, Che, J, Cheblal, A, Elango, R, Epshtein, A, Fitzgerald, D M, Gómez-González, B, Khan, S R, Kumar, S, Leland, B A, Marie, L, Mei, Q, Miné-Hattab, J, Piotrowska, A, Polleys, E J, Putnam, C D, Radchenko, E A, Saada, A A, Sakofsky, C J, Shim, E Y, Stracy, M, Xia, J, Yan, Z, Yin, Y, Aguilera, A, Argueso, J L, Freudenreich, C H, Gasser, S M, Gordenin, D A, Haber, J E, Ira, G, Jinks-Robertson, S, King, M C, Kolodner, R D, Kuzminov, A, Lambert, S A E, Lee, S E, Miller, K M, Mirkin, S M, Petes, T D, Rosenberg, S M, Rothstein, R, Symington, L S, Zawadzki, P, Kim, N, Lisby, M & Malkova, A 2019, ' Guidelines for DNA recombination and repair studies : cellular assays of DNA repair pathways ', Microbial Cell Factories, vol. 6, no. 1, pp. 1-64 . https://doi.org/10.15698/mic2019.01.664 |
| ISSN: | 2311-2638 |
| Popis: | 2019 Klein et al. Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies. HLK was supported by NIH grant R01-CA146940. AA was supported by the European research Council (ERC2014- ADG669898 TARLOOP), the Spanish Ministry of Economy and Competitiveness (BFU2016-75058-P), and the Junta de Andalucía (BIO1238). JLA was supported by NIH grant R35G-GM119788. CHF was supported by NIH grants P01- GM105473 and R01-GM122880. SMG was supported by the Swiss national Science Foundation and the Novartis Research Foundation. DAG was supported by National Institutes of Health (Intramural Research Program Project Z1AES103266. JEH was supported by NIH grants R35- GM127029 and P01-GM105473. GI was supported by NIH grants R01-GM125650 and R01-GM080600. SJ-R was supported by NIH grant R35-GM118077. MCK was supported by National Science Foundation Graduate Research Fellowship DGE-1122492, NIH training grant T32-GM007223 and NIH grant DP20D008429. RDK and CDP were supported by NIH grants R01-GM26017 and R01-GM50006. AK was supported by NIH grant R01-GM073115. SAEL was supported by grants from Agence Nationale de la Recherche ANR-14- CE10-0010-01 and the Foundation pour la Recherche Médicale Equipe FRM DEQ20160334889. SEL was supported by NIH grant R01-GM071011. SMM was supported by NIH grants R01-GM60987 and P01-GM105473. TDP was supported by NIH grant R35-GM118020. SMR was supported by a gift from WM Keck Foundation, NIH Director’s Pioneer Award DP1-CA174424 and NIH grant R35-GM122598. DMF was supported by the Cancer Prevention and Research Institute of Texas, Baylor College of Medicine Comprehensive Cancer Training Program Postdoctoral Fellowship RP160283. RR was supported by NIH grant R35-GM118180 and JM-H was supported by a Marie Curie International Outgoing Fellowship and the ANR-12-PDOC-0035-01. LSS was supported by NIH grant R35-GM126997. PZ was supported by National Science Centre Poland 2015/19/103859 and FNP First TEAM/2016-1/9. NK was supported by NIH grant R01-GM116007 and the Welch Foundation (AU1875). ML was supported by grants from the Danish Agency for Science, Technology and Innovation, the Villum Foundation and the Danish National Research Foundation (DNRF115). AM was supported by NIH grants R35-GM127006 and R03- ES029306. |
| Databáze: | OpenAIRE |
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