Human renal-cell carcinoma cells are able to activate natural killer cells
| Autor: | Kyogo Itoh, K. Hayakawa, Tatsuo Morita, Andrew C. von Eschenbach, Lazel B. Augustus |
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| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Receptors Fc Lymphocyte Activation urologic and male genital diseases Natural killer cell Interleukin 21 Lymphocytes Tumor-Infiltrating NK-92 Tumor Cells Cultured medicine Humans IL-2 receptor Carcinoma Renal Cell neoplasms Lymphokine-activated killer cell CD40 biology Receptors IgG Receptors Interleukin-2 HLA-DR Antigens Natural killer T cell Antigens Differentiation Molecular biology Kidney Neoplasms female genital diseases and pregnancy complications Killer Cells Natural medicine.anatomical_structure Oncology Immunology Interleukin 12 biology.protein |
| Zdroj: | International Journal of Cancer. 51:290-295 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910510219 |
| Popis: | We previously reported that natural killer (NK) cells that had infiltrated renal-cell carcinoma (RCC) proliferated vigorously in culture with interleukin-2 (IL-2) and lysed autologous tumor cells. In this study, we investigate the susceptibility of RCC cells to NK-cell lysis and their ability to stimulate proliferation and increase phenotypic expression and function of NK cells. Cells from primary culture of RCC (p-RCC cells) were significantly more susceptible to the lysis mediated by human NK3.3 clones than were cells from primary culture of metastatic melanomas. Both RCC-cell clones and cells from primary culture of non-tumorous kidneys were also susceptible to lysis by NK3.3 clones and IL-2-activated peripheral blood lymphocytes (PBLs). Incubation of NK3.3 clones with p-RCC cells in the absence of IL-2 induced proliferation of NK3.3 clones, whereas incubation with cells from primary culture of metastatic melanomas, K562 cells, or any others tested did not. The p-RCC cells from earlier passages were more potent inducers of NK-cell proliferation than were those from older passages. Cell-free culture supernatants of p-RCC cells with or without NK3.3 clones failed to induce NK-cell proliferation. Incubation of CD16+ NK cells purified from PBLs with p-RCC cells induced higher proliferation of the NK cells only in the presence of IL-2, whereas incubation with cells from primary culture of metastatic melanomas did not. Incubation of NK3.3 clones with p-RCC cells resulted in an increase in CD16, CD25 (IL-2 receptor-alpha), and HLA-DR antigen expression and cytotoxicity in NK3.3 clones. In summary, these results suggest that RCC cells are able to activate NK cells, potentially through cell-to-cell interaction. |
| Databáze: | OpenAIRE |
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