Identification and characterisation of side population cells in the canine pituitary gland
| Autor: | van Rijn, Sarah J, Gremeaux, Lies, Riemers, Frank M, Brinkhof, Bas, Vankelecom, Hugo, Penning, Louis C, Meij, Björn P, Opleiding chirurgie, Biochemisch laboratorium, Sub Celbiologisch lab., Onderzoek, LS Algemene chirurgie, Sub Neuro Chirurgie |
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| Přispěvatelé: | Opleiding chirurgie, Biochemisch laboratorium, Sub Celbiologisch lab., Onderzoek, LS Algemene chirurgie, Sub Neuro Chirurgie |
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pituitary gland Population CD34 Biology Flow cytometry Dogs Side population Anterior pituitary Internal medicine medicine Animals Progenitor cell education Side-Population Cells education.field_of_study General Veterinary medicine.diagnostic_test Flow Cytometry Molecular biology Endocrinology medicine.anatomical_structure Gene Expression Regulation Pituitary Gland Animal Science and Zoology Stem cell |
| Zdroj: | Veterinary Journal, 192(3), 476. Bailliere Tindall Ltd |
| ISSN: | 1532-2971 1090-0233 |
| Popis: | To date, stem/progenitor cells have not been identified in the canine pituitary gland. Cells that efficiently exclude the vital dye Hoechst 33342 can be visualised and identified using fluorescence activated cell sorting (FACS) as a 'side population' (SP), distinct from the main population (MP). Such SPs have been identified in several tissues and display stem/progenitor cell characteristics. In this study, a small SP (1.3%, n=6) was detected in the anterior pituitary glands of healthy dogs. Quantitative PCR indicated significantly higher expression of CD34 and Thy1 in this SP, but no differences in the expression of CD133, Bmi-1, Axin2 or Shh. Pro-opiomelanocortin (POMC) and Lhx3 expression were significantly higher in the MP than in the SP, but no differences in the expression of Tpit, GH or PRL were found. The study demonstrated the existence of an SP of cells in the normal canine pituitary gland, encompassing cells with stem cell characteristics and without POMC expression. |
| Databáze: | OpenAIRE |
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