Amphetamine Activates an Amine-gated Chloride Channel to Generate Behavioral Effects in Caenorhabditis elegans
| Autor: | Laura Bianchi, Lucia Carvelli, Chee Lor, Bryan D. Safratowich |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Patch-Clamp Techniques
Time Factors Dopamine Plasma Membrane Transport Proteins Sodium Chloride Pharmacology Biochemistry Membrane Potentials Potassium Chloride Gene Knockout Techniques Xenopus laevis Neurobiology Chloride Channels Receptors Biogenic Amine Dopamine parasitic diseases mental disorders medicine Animals Amines Caenorhabditis elegans Caenorhabditis elegans Proteins Amphetamine Molecular Biology Dopamine transporter Dose-Response Relationship Drug biology food and beverages Transporter Cell Biology biology.organism_classification Electrophysiology nervous system Oocytes Chloride channel biology.protein Central Nervous System Stimulants Female Ion Channel Gating Neuroscience medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 288:21630-21637 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m113.484139 |
| Popis: | Amphetamine is a highly addictive psychostimulant, which is thought to generate its effects by promoting release of dopamine through reverse activation of dopamine transporters. However, some amphetamine-mediated behaviors persist in dopamine transporter knock-out animals, suggesting the existence of alternative amphetamine targets. Here we demonstrate the identification of a novel amphetamine target by showing that in Caenorhabditis elegans, a large fraction of the behavioral effects of amphetamine is mediated through activation of the amine-gated chloride channel, LGC-55. These findings bring to light alternative pathways engaged by amphetamine, and urge rethinking of the molecular mechanisms underlying the effects of this highly-addictive psychostimulant. |
| Databáze: | OpenAIRE |
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