Genome-wide analysis identifies chickpea (Cicer arietinum) heat stress transcription factors (Hsfs) responsive to heat stress at the pod development stage
| Autor: | Prasanth Tej Kumar Jagannadham, Santosh Halasabala Basavarajappa, D.K. Kohli, Jitendra Kumar, Ramamurthy Srinivasan, Bharadwaj Chellapilla, Pradeep K. Jain, Parameswaran Chidambaranathan, Viswanathan Satheesh |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Candidate gene Hot Temperature In silico Plant Science Biology 01 natural sciences Genome 03 medical and health sciences chemistry.chemical_compound Heat Shock Transcription Factors Stress Physiological Gene Duplication Botany Gene family Amino Acid Sequence Gene Abscisic acid Phylogeny Plant Proteins Genetics Plant physiology Cicer Droughts 030104 developmental biology Point of delivery chemistry Sequence Alignment Genome Plant Heat-Shock Response 010606 plant biology & botany |
| Zdroj: | Journal of Plant Research. 131:525-542 |
| ISSN: | 1618-0860 0918-9440 |
| DOI: | 10.1007/s10265-017-0948-y |
| Popis: | The heat stress transcription factors (Hsfs) play a prominent role in thermotolerance and eliciting the heat stress response in plants. Identification and expression analysis of Hsfs gene family members in chickpea would provide valuable information on heat stress responsive Hsfs. A genome-wide analysis of Hsfs gene family resulted in the identification of 22 Hsf genes in chickpea in both desi and kabuli genome. Phylogenetic analysis distinctly separated 12 A, 9 B, and 1 C class Hsfs, respectively. An analysis of cis-regulatory elements in the upstream region of the genes identified many stress responsive elements such as heat stress elements (HSE), abscisic acid responsive element (ABRE) etc. In silico expression analysis showed nine and three Hsfs were also expressed in drought and salinity stresses, respectively. Q-PCR expression analysis of Hsfs under heat stress at pod development and at 15 days old seedling stage showed that CarHsfA2, A6, and B2 were significantly upregulated in both the stages of crop growth and other four Hsfs (CarHsfA2, A6a, A6c, B2a) showed early transcriptional upregulation for heat stress at seedling stage of chickpea. These subclasses of Hsfs identified in this study can be further evaluated as candidate genes in the characterization of heat stress response in chickpea. |
| Databáze: | OpenAIRE |
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