Assessment of cross-reactivity among five species of house dust and storage mites
| Autor: | Rania Farmaki, Rosanna Marsella, Robert E. Esch, Alexander F. Koutinas, Kenneth W. Lee, Manolis N. Saridomichelakis |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Veterinary medicine Enzyme-Linked Immunosorbent Assay Cross Reactions medicine.disease_cause Immunoglobulin E Sensitivity and Specificity Cross-reactivity Tyrophagus putrescentiae Group A Group B Dermatitis Atopic Serology Dogs Species Specificity medicine Animals False Positive Reactions Antigens Dermatophagoides Dog Diseases Acaridae General Veterinary biology Pyroglyphidae Atopic dermatitis medicine.disease Acarus biology.organism_classification respiratory tract diseases biology.protein Female |
| Zdroj: | Veterinary Dermatology. 19:67-76 |
| ISSN: | 1365-3164 0959-4493 |
| DOI: | 10.1111/j.1365-3164.2008.00654.x |
| Popis: | In vitro cross-reactivity among two house dust (Dermatophagoides farinae, D. pteronyssinus) and three storage (Acarus siro, Tyrophagus putrescentiae, Lepidoglyphus destructor) mites was examined in 20 mite-sensitive dogs with natural occurring atopic dermatitis (group A), 13 high-IgE beagles experimentally sensitized to D. farinae (group B), and five healthy beagles (group C). Intradermal testing (IDT) and serology for allergen-specific IgE demonstrated that co-sensitization for all possible pairs of the five mites was generally 45% or higher among group A dogs. In the same dogs, enzyme-linked immunosorbent assay cross-inhibition results indicated that each one of D. farinae, A. siro and T. putrescentiae was a strong inhibitor of all the remaining mites, whereas D. pteronyssinus was a strong inhibitor of L. destructor. A high number of positive IDT and serology test results for D. pteronyssinus, A. siro, T. putrescentiae and L. destructor were recorded among group B dogs. No conclusive evidence of exposure to these mites was found upon analysis of dust samples from their environment and their food for the presence of mites and guanine. Also, the number of positive test results was generally higher among group B than among group C dogs. Enzyme-linked immunosorbent assay cross-inhibition revealed that D. farinae was a strong inhibitor of D. pteronyssinus, A. siro and T. putrescentiae. Collectively, these results demonstrated extensive in vitro cross-reactivity among house dust and/or storage mites that can explain false-positive results upon testing of dust mite-sensitive dogs with atopic dermatitis. |
| Databáze: | OpenAIRE |
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