Secretion of adiponectin multimeric complexes from adipose tissue explants is not modified by very low calorie diet
| Autor: | Lenka Rossmeislová, Zuzana Hnevkovska, Magda Bajzova, Zuzana Kovacova, Jan Polak, Michaela Vitkova, Dominique Langin, Vladimir Stich, Michaela Kovacikova, Eva Klimcakova |
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| Přispěvatelé: | Sports Medicine Department, Third Faculty of Medicine Charles University, Division of Cell and Molecular Biology, Franco-Czech Laboratory for Clinical Research on Obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Internal Medicine Department, Vinohrady Teatching Hospital, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biochimie [Purpan], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut Fédératif de Biologie (IFB) - Hôpital Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Simon, Marie Francoise, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Luminescence Polymers Endocrinology Diabetes and Metabolism Adipose tissue Tissue Culture Techniques 0302 clinical medicine Endocrinology Weight loss Blood plasma MESH: Obesity 2. Zero hunger 0303 health sciences medicine.diagnostic_test MESH: Kinetics MESH: Luminescence MESH: Molecular Weight MESH: Waist-Hip Ratio Biopsy Needle MESH: Enzyme-Linked Immunosorbent Assay General Medicine MESH: Adiponectin 3. Good health MESH: Polymers Very low calorie diet Adipose Tissue Female Adiponectin medicine.symptom MESH: Adipose Tissue Adult medicine.medical_specialty MESH: Biopsy Needle food.diet Blotting Western 030209 endocrinology & metabolism Enzyme-Linked Immunosorbent Assay Biology 03 medical and health sciences MESH: Weight Loss food Western blot Internal medicine MESH: Body Height Weight Loss medicine Humans MESH: Blotting Western Secretion Obesity MESH: Tissue Culture Techniques 030304 developmental biology Caloric Restriction MESH: Caloric Restriction MESH: Humans Waist-Hip Ratio Body Weight MESH: Adult medicine.disease Body Height MESH: Male Culture Media MESH: Body Weight Molecular Weight Kinetics MESH: Culture Media MESH: Female |
| Zdroj: | European Journal of Endocrinology European Journal of Endocrinology, BioScientifica, 2009, 160 (4), pp.585-92. ⟨10.1530/EJE-08-0727⟩ European Journal of Endocrinology, 2009, 160 (4), pp.585-92. ⟨10.1530/EJE-08-0727⟩ |
| ISSN: | 0804-4643 1479-683X |
| Popis: | ObjectiveAdiponectin is a protein abundantly secreted by the adipose tissue (AT). Plasma adiponectin levels are decreased in obese, insulin-resistant, and type 2 diabetic patients. Various multimeric complexes, i.e. high-, middle-, and low-molecular weight isoforms (HMW, MMW and LMW), are present in plasma. Here, we investigated the effect of weight reducing diet on the distribution of adiponectin isoforms in plasma and on their secretion in AT explants from obese subjects.DesignA total of 20 obese subjects (age 37.8±7.3 years, body mass index 33.9±5.0 kg/m2) underwent eight weeks of very low-calorie diet (VLCD). A needle biopsy of subcutaneous abdominal AT and blood samples were taken before and after dietary intervention. AT explants were incubated in culture medium for 4 h. ELISA assay and western blot analyses were used to identify adiponectin complexes in culture media and in plasma.ResultsThe distribution of adiponectin polymers in plasma was different from that secreted in human AT explants. Before VLCD, the relative amount of HMW isoform was 75.5±9.1% of total adiponectin in culture media and 52.2±11.2% in plasma. Despite the diet-induced weight loss and improvement of insulin sensitivity, VLCD neither induced change in total adiponectin level nor in the ratio of HMW to total adiponectin in plasma and in culture media of AT explants.ConclusionsThe profile of adiponectin polymeric isoforms secreted by AT explants into culture media differs from the plasma profile. A dietary intervention leading to weight loss and improvement of insulin sensitivity was not associated with modifications of AT secretion of total or HMW adiponectin. |
| Databáze: | OpenAIRE |
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