A gold@silica core–shell nanoparticle-based surface-enhanced Raman scattering biosensor for label-free glucose detection
| Autor: | Alice K. Melconian, Israa Al-Ogaidi, Nianqiang Wu, Peng Zheng, Abdul Kareem A. Al-kazaz, Honglei Gou, Zoraida P. Aguilar |
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| Rok vydání: | 2014 |
| Předmět: |
Silicon dioxide
Inorganic chemistry Metal Nanoparticles Nanoparticle Ascorbic Acid Biosensing Techniques Spectrum Analysis Raman Gluconates Biochemistry Chemistry Techniques Analytical Analytical Chemistry Glucose Oxidase chemistry.chemical_compound Environmental Chemistry Glucose oxidase Hydrogen peroxide Spectroscopy Detection limit biology Hydrogen Peroxide Enzymes Immobilized Silicon Dioxide Ascorbic acid Uric Acid Glucose chemistry biology.protein Gold Fluorescent glucose biosensor Oxidation-Reduction Biosensor |
| Zdroj: | Analytica Chimica Acta. 811:76-80 |
| ISSN: | 0003-2670 |
| Popis: | The gold nanostar@silica core-shell nanoparticles conjugated with glucose oxidase (GOx) enzyme molecules have been developed as the surface-enhanced Raman scattering (SERS) biosensor for label-free detection of glucose. The surface-immobilized GOx enzyme catalyzes the oxidation of glucose, producing hydrogen peroxide. Under laser excitation, the produced H2O2 molecules near the Au nanostar@silica nanoparticles generate a strong SERS signal, which is used to measure the glucose concentration. The SERS signal of nanostar@silica∼GOx nanoparticle-based sensing assay shows the dynamic response to the glucose concentration range from 25 μM to 25 mM in the aqueous solution with the limit of detection of 16 μM. The sensing assay does not show any interference when glucose co-exists with both ascorbic acid and uric acid. The sensor can be applied to a saliva sample. |
| Databáze: | OpenAIRE |
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