The expression of serotonin receptors by cultured rat Schwann cells is a function of their differentiation: correlation with a quiescent myelinating phenotype
| Autor: | Barron Lee, Mark H. Ellisman, Elizabeth J. Yoder |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Serotonin
Cell division Cellular differentiation Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Immunolabeling Animals Receptor Molecular Biology 5-HT receptor Cells Cultured Myelin Sheath biology Osmolar Concentration Cell Differentiation Cell Biology Fetal Blood Molecular biology Rats Phenotype chemistry Receptors Serotonin biology.protein Cattle Schwann Cells Antibody Bromodeoxyuridine Biomarkers Cell Division |
| Zdroj: | Molecular and cellular neurosciences. 8(5) |
| ISSN: | 1044-7431 |
| Popis: | Previously we reported that cultured rat Schwann cells express 5-HT2A receptors. In the present study we compared the serotonin-responsiveness of Schwann cells with their stage of differentiation. Serotonin-responsiveness occurred in cells that expressed a myelinating marker but not a nonmyelinating marker. We also examined cells to see if those responding to 5-HT were selectively proliferating or quiescent. This was accomplished by pulsing cells with bromodeoxyuridine (BrdUrd), screening them for serotonin responses, and then immunolabeling the same cells with an antibody recognizing BrdUrd. No BrdUrd+ cells responded to serotonin, whereas most BrdUrd- cells did respond, indicating that serotonin receptors are expressed in Schwann cells after they become quiescent. Moreover, detection of the responses is enhanced by reducing concentrations of mitogen-containing serum in the culture medium. Collectively, these results suggest that postmitotic Schwann cells express serotonin receptors while differentiating along the myelinating lineage. |
| Databáze: | OpenAIRE |
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