The effect of axotomy and denervation on calmodulin content in the superior cervical sympathetic ganglion of the rat
| Autor: | Yutaka Nagata, Mamoru Sano, Akiko Seto-Ohshima, Yoshiro Yamazaki, Noriko Kawamura, Satoko Kitajima |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Superior cervical ganglion medicine.medical_specialty Time Factors animal structures Autonomic Fibers Preganglionic medicine.medical_treatment In Vitro Techniques Immunoenzyme Techniques Calmodulin Internal medicine medicine Animals Molecular Biology Denervation Retina Ganglia Sympathetic Histocytochemistry business.industry General Neuroscience Rats Inbred Strains Nodose Ganglion Anatomy Axons Rats Ganglion Microscopy Electron medicine.anatomical_structure Endocrinology Autonomic Fibers Postganglionic Immunohistochemistry Female sense organs Neurology (clinical) Axotomy business Immunostaining Adenylyl Cyclases Developmental Biology |
| Zdroj: | Brain Research. 410:292-298 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(87)90327-1 |
| Popis: | Calmodulin (CaM) in the superior cervical sympathetic ganglion (SCG) of the rat and its changes after transection of the pre- or postganglionic nerves were studied biochemically and immunohistochemically. The concentration of CaM in the SCG was assayed using the extent of activation of CaM-dependent adenylate cyclase from bovine neural retina. In the SCG, CaM concentration was 4.5 micrograms/mg protein, a level similar to that in another peripheral ganglion, the nodose ganglion (4.9 micrograms/mg protein). Following denervation of the SCG, the total amount of ganglionic CaM did not change significantly within a week. On the other hand, CaM content per ganglion increased gradually 3 days after axotomy. Immunohistochemical examination revealed that CaM in unoperated SCG is present both in the neuronal fibers and in the cell soma of sympathetic neurons. But the intensity of the staining with CaM-antibody in the cytoplasmic soma varied among cells. The gross profile of the CaM immunostaining seemed to be unchanged one week after either denervation or axotomy, but the immunoelectron microscopic study showed heavy staining of the innercellular membranes of irregular shape in the axotomized SCG. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|