An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae
| Autor: | Yasuo Yoshikuni, David Baker, Ryan M. Raisner, Asael Herman, Yuki Kashiyama, Effendi Leonard, Maung Nyan Win, Alicia B. Sivitz, Peter B Kim, Christine Nicole S. Santos, Drew D. Regitsky, Susan Rudd Cooper, Arun Lakshmanaswamy, Adam J. Wargacki |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Alginates
Bioconversion Commodity chemicals Microorganism Biology Phaeophyta Polysaccharide Open Reading Frames Bacterial Proteins Glucuronic Acid Botany Escherichia coli Mannitol Biomass Lactic Acid Food science Sugar Polysaccharide-Lyases Vibrio chemistry.chemical_classification Multidisciplinary Ethanol Depolymerization Hexuronic Acids Biological Transport Seaweed Glucose Metabolic Engineering chemistry Genes Bacterial Biofuel Biofuels Yield (chemistry) Fermentation Carrier Proteins Metabolic Networks and Pathways |
| Zdroj: | Science. 335:308-313 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio splendidus encoding enzymes for alginate transport and metabolism. The genomic integration of this ensemble, together with an engineered system for extracellular alginate depolymerization, generated a microbial platform that can simultaneously degrade, uptake, and metabolize alginate. When further engineered for ethanol synthesis, this platform enables bioethanol production directly from macroalgae via a consolidated process, achieving a titer of 4.7% volume/volume and a yield of 0.281 weight ethanol/weight dry macroalgae (equivalent to ~80% of the maximum theoretical yield from the sugar composition in macroalgae). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|