Antiparasitic potential of ethanolic extracts of Carpesii Fructus against Miamiensis avidus in hirame natural embryo cell line and their effects on immune response- and biotransformation-related genes
| Autor: | Eun Ji Jeon, Na Young Kim, Mi Young Do, Min Gyeong Jeong, Soo Ji Woo |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Lysis
Dried fruit Physiology Antiparasitic medicine.drug_class Parasitic Diseases Animal Health Toxicology and Mutagenesis Flounder Asteraceae Pharmacology Toxicology Biochemistry Cell Line Fish Diseases Immune system medicine Animals Antiparasitic Agents biology Plant Extracts Immunity Embryo Cell Biology General Medicine Antiparasitic agent In vitro Concanavalin A biology.protein |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 251:109214 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2021.109214 |
| Popis: | Scuticociliatosis, caused by Miamiensis avidus, is a severe parasitic disease affecting marine organisms, particularly Paralichthys olivaceus. The aim of this study was to assess the antiparasitic potential of ethanolic extracts of Carpesii Fructus (EECF), the dried fruit of Carpesium abrotanoides L., which is used in traditional Chinese medicine, in vitro. We found that 50%, 70%, and 100% EECF induced morphological changes in M. avidus, including reduced motility, cell shrinkage, and lysis. Nearly 100% cell lysis was observed in M. avidus after 2 h of treating with 100% EECF. After 24 h, the survival rates of M. avidus treated with 100%, 70%, and 50% EECF were 10%, 20%, and 30%, respectively. Additionally, the mRNA levels of immune response-related (IL-1β, IL-8, TNF-α, and CD8-α) and biotransformation-related (CYP1A, CYP1B, CYP3A4, and UGT2B19) genes increased with 70% and 100% EECF treatment and decreased with 50% EECF treatment following pretreatment with concanavalin A. The viability of hirame natural embryo (HINAE) cells was reduced by 50%, 70%, and 100% EECF (100 mg/L) and was between 67 and 80%. The IC50 values of 50%, 70%, 90%, and 100% EECF in HINAE cells were 102.3, 42.93, 39.15, and 38.39 mg/L, respectively. These results indicated that 50% EECF was less toxic to HINAE cells than 70% or 100% EECF, while still exhibiting antiparasitic activity against M. avidus. Therefore, we demonstrated the role of EECF as a natural antiparasitic agent against M. avidus. Our findings suggest that Carpesii Fructus has potential use as an antiparasitic agent in the aquaculture industry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect