Description of the growth hormone gene of the Australasian snapper, Chrysophrys auratus , and associated intra‐ and interspecific genetic variation
| Autor: | Kate Irving, Maren Wellenreuther, Peter A. Ritchie |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Population Aquatic Science 010603 evolutionary biology 01 natural sciences Exon Genetic variation Animals Coding region education Gene Phylogeny Ecology Evolution Behavior and Systematics education.field_of_study Phylogenetic tree biology 010604 marine biology & hydrobiology Fishes Intron Genetic Variation biology.organism_classification Introns Chrysophrys auratus Perciformes Evolutionary biology Growth Hormone |
| Zdroj: | Journal of Fish Biology. 99:1060-1070 |
| ISSN: | 1095-8649 0022-1112 |
| DOI: | 10.1111/jfb.14810 |
| Popis: | The growth hormone (GH) gene of the marine teleost, the Australasian snapper (Chrysophrys auratus), was identified and characterized from the reference genome showing it was approximately 5577 bp in length and consisted of six exons and five introns. Large polymorphic repeat regions were found in the first and third introns, and putative transcription factor binding sites were identified. Phylogenetic analysis of the GH genes of perciform fish showed largely conserved coding regions and highly variable noncoding regions among species. Despite some exon sequence variation and an amino acid deletion identified between C. auratus and its sister species Chrysophrys/Pagrus major, the amino acid sequences and putative secondary structures were largely conserved across the Sparidae. A population-level assessment of 99 samples caught at five separate coastal locations in New Zealand revealed six variable alleles at the intron 1 site of the C. auratus GH gene. A population genetic analysis suggested that C. auratus from the five sample locations were largely panmictic, with no evidence for departure from the Hardy-Weinberg equilibrium, and have a high level of heterozygosity. Overall these results suggest that the GH gene is largely conserved across the coding regions, but some variability could be detected. |
| Databáze: | OpenAIRE |
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