Gallbladder interstitial Cajal-like cells and gallbladder contractility in patients with cholelithiasis: a prospective study
| Autor: | Junmin Wei, Runyu Ding, Jingyong Xu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male medicine.medical_specialty Histology Gallbladder Emptying medicine.medical_treatment Tryptase Cell Count Gastroenterology Antibodies Pathology and Forensic Medicine Contractility Pathogenesis 03 medical and health sciences Mice 0302 clinical medicine Cholelithiasis Internal medicine medicine Animals Humans In patient Prospective Studies Telocytes Prospective cohort study Interstitial Cajal-Like Cells Aged biology business.industry Gallbladder Goats General Medicine Middle Aged Proto-Oncogene Proteins c-kit medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Cholecystectomy Female Tryptases Rabbits business |
| Zdroj: | Folia histochemica et cytobiologica. 57(2) |
| ISSN: | 1897-5631 |
| Popis: | Introduction. A reduced number of interstitial Cajal-like cells (ICLCs) in the gallbladder have been proposed to play a role in the pathogenesis of cholelithiasis. Therefore, this prospective study was conducted to investigate the relationship between gallbladder contractility and the number of gallbladder ICLCs in patients with cholelithiasis. Material and methods. Patients admitted to the Department of Hepatobiliary Surgery for cholecystectomy were divided into the cholelithiasis (n = 18) and non-cholelithiasis (n = 8) groups based on their clinical data. Patients’ clinical data were collected on admission, and B-mode ultrasonography was performed to assess their gallbladder contractility. The resected gallbladder specimens were fixed, paraffin sections mounted on slides, and the immunofluorescence staining with the anti-human CD-117 and anti-human tryptase antibodies was performed to identify ICLSs and mast cells, respectively. The number of ICLCs was counted in 10 high-power fields (HPFs) randomly. Results. Independent sample t-tests revealed differences between the cholelithiasis and non-cholelithiasis groups in the number of ICLCs (mean ± standard deviation: 88.61 ± 28.22 vs. 115.89 ± 27.87 per HPFs, P = 0.032) and gallbladder contractility (43.94% ± 18.50% vs. 61.00% ± 20.50%, P = 0.046). Pearson and Spearman correlation analyses revealed no significant correlation between the number of ICLCs and gallbladder contractility. Conclusion. The results suggest that the number of gallbladder ICLCs in the wall of the gallbladder of patients with or without cholelithiasis is not a decisive factor affecting gallbladder contractility. |
| Databáze: | OpenAIRE |
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