Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation
| Autor: | Mingyu Yang, Yin Chen, Helge-Ansgar Giebel, Mathias Wolterink, David J. Scanlan, Yanlin Zhao, Eleonora Silvano, Meinhard Simon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Ruegeria lcsh:QR1-502 Heterotroph Glycerophospholipids Microbiology lcsh:Microbiology lipids QH301 03 medical and health sciences chemistry.chemical_compound Biosynthesis 14. Life underwater 030304 developmental biology Original Research Phosphatidylethanolamine 0303 health sciences biology roseobacter clade bacteria 030306 microbiology Ruegeria pomeroyi DSS-3 Bacterioplankton lipid remodeling Roseobacter biology.organism_classification chemistry Biochemistry Bacteria marine heterotrophic bacteria |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2020) |
| ISSN: | 1664-302X |
| Popis: | The marine roseobacter-clade Affiliated cluster (RCA) represents one of the most abundant groups of bacterioplankton in the global oceans, particularly in temperate and sub-polar regions. They play a key role in the biogeochemical cycling of various elements and are important players in oceanic climate-active trace gas metabolism. In contrast to copiotrophic roseobacter counterparts such as Ruegeria pomeroyi DSS-3 and Phaeobacter sp. MED193, RCA bacteria are truly pelagic and have smaller genomes. We have previously shown that RCA bacteria do not appear to encode the PlcP-mediated lipid remodelling pathway, whereby marine heterotrophic bacteria remodel their membrane lipid composition in response to phosphorus (P) stress by substituting membrane glycerophospholipids with alternative glycolipids or betaine lipids. In this study, we report lipidomic analysis of six RCA isolates. In addition to the commonly found glycerophospholipids such as phosphatidylglycerol and phosphatidylethanolamine, RCA bacteria synthesise a relatively uncommon phospholipid, acylphosphatidylglycerol, which is not found in copiotrophic roseobacters. Instead, like the abundant SAR11 clade, RCA bacteria upregulate ornithine lipid biosynthesis in response to P stress, suggesting a key role of this aminolipid in the adaptation of marine heterotrophs to oceanic nutrient limitation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|