BCR-ABL fusion protein detection in peripheral blood and bone marrow samples of adult precursor B-cell acute lymphoblastic leukemia patients using the flow cytometric immunobead assay
| Autor: | Katerina Psarra, Eirini Grigoriou, Eirini Tziotziou, C. Papasteriades, Maria Garofalaki |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Clinical Biochemistry Fusion Proteins bcr-abl Polymerase Chain Reaction law.invention Flow cytometry Fusion gene Young Adult law Bone Marrow hemic and lymphatic diseases medicine Humans Polymerase chain reaction In Situ Hybridization Fluorescence Aged Aged 80 and over Immunoassay medicine.diagnostic_test business.industry Biochemistry (medical) Cytogenetics General Medicine Middle Aged Precursor Cell Lymphoblastic Leukemia-Lymphoma Flow Cytometry Molecular biology Fusion protein Peripheral blood medicine.anatomical_structure ROC Curve Immunology Female Bone marrow business Fluorescence in situ hybridization |
| Zdroj: | Clinical chemistry and laboratory medicine. 50(9) |
| ISSN: | 1437-4331 |
| Popis: | BACKGROUND The ability to detect the BCR-ABL fusion gene in precursor B-cell acute lymphoblastic leukemia (pB-ALL) is essential for making accurate treatment decisions. METHODS We used a new flow cytometric immunobead assay for BCR-ABL fusion protein detection in peripheral blood and/or bone marrow samples from 38 adult pB-ALL patients and the results were compared with polymerase chain reaction (PCR) detection of BCR-ABL transcript. RESULTS The fusion protein was detected in peripheral blood and bone marrow samples from seven of the 38 (18%) patients, and results for both the p190 and p210 were confirmed by PCR. One case, which was positive by cytogenetics and fluorescence in situ hybridization (FISH), was negative by PCR but positive by flow cytometry. Another case, which was positive by PCR and negative by flow cytometry, was from a patient on steroid treatment. CONCLUSIONS The cytometric immunobead assay for BCR-ABL fusion protein detection was found to be suitable for the investigation of pB-ALL patients. This assay is reliable, rapid and simple to use for peripheral blood and bone marrow samples. |
| Databáze: | OpenAIRE |
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