Class II-restricted T cell responses in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease
| Autor: | Jeffrey D. Peterson, Vincent K. Tuohy, Carl Waltenbaugh, John L. Trotter, Howard L. Lipton, Mary K. Kennedy, S J Gerety, Stephen D. Miller, Mauro C. Dal Canto |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Proteolipid protein 1
biology Encephalomyelitis Multiple sclerosis T cell Immunology Clinical Neurology medicine.disease Virology nervous system diseases Myelin basic protein Immune tolerance Myelin medicine.anatomical_structure Neurology immune system diseases medicine biology.protein Demyelinating disease Immunology and Allergy Neurology (clinical) |
| Zdroj: | Journal of Neuroimmunology |
| ISSN: | 0165-5728 |
| DOI: | 10.1016/0165-5728(90)90115-4 |
| Popis: | Intracerebral inoculation of Theiler's murine encephalomyelitis virus (TMEV) into susceptible mouse strains produces a chronic demyelinating disease in which mononuclear cell-rich infiltrates in the central nervous system (CNS) are prominent. Current evidence strongly supports an immune-mediated basis for myelin breakdown, with an effector role proposed for TMEV-specific, major histocompatibility complex (MHC) class II-restricted delayed-type hypersensitivity (DTH) responses in which lymphokine-activated macrophages mediate bystander demyelination. The present study examined the possibility that concomitant or later-appearing neuroantigen-specific autoimmune T cell responses, such as those demonstrated in chronic-relapsing experimental allergic encephalomyelitis (R-EAE), may contribute to the demyelinating process following TMEV infection. T cell responses against intact, purified major myelin proteins (myelin basic protein (MBP) and proteolipid protein (PLP], and against altered myelin constituents were readily demonstrable in SJL/J mice with R-EAE, but were not detectable in SJL/J mice with TMEV-induced demyelinating disease. TMEV-infected mice also did not display T cell responses against the peptide fragments of MBP(91-104) and PLP(139-151) recently shown to be encephalitogenic in SJL/J mice. In addition, induction of neuroantigen-specific tolerance to a heterogeneous mixture of CNS antigens, via the i.v. injection of syngeneic SJL/J splenocytes covalently coupled with mouse spinal cord homogenate, resulted in significant suppression of clinical and histologic signs of R-EAE and the accompanying MBP- and PLP-specific DTH responses. In contrast, neuroantigen-specific tolerance failed to alter the development of clinical and histologic signs of TMEV-induced demyelinating disease or the accompanying virus-specific DTH and humoral immune responses. These findings demonstrate that TMEV-induced demyelinating disease can occur in the apparent absence of neuroantigen-specific autoimmune responses. The relationship of the present results to the immunopathology of multiple sclerosis is discussed. |
| Databáze: | OpenAIRE |
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