Amperometric aptasensor for carcinoembryonic antigen based on the use of bifunctionalized Janus nanoparticles as biorecognition-signaling element
| Autor: | Borja Vegas, Gustavo A. Rivas, Reynaldo Villalonga, Concepción Parrado, Anabel Villalonga, Marcos Eguílaz, Gonzalo Paniagua, Paula Díez |
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| Rok vydání: | 2018 |
| Předmět: |
Surface Properties
Aptamer Nanoparticle Nanotechnology 02 engineering and technology Biosensing Techniques 01 natural sciences Biochemistry Horseradish peroxidase Analytical Chemistry chemistry.chemical_compound Biotin Environmental Chemistry Nanoparticle Complex Particle Size Spectroscopy Detection limit biology Chemistry 010401 analytical chemistry Electrochemical Techniques Aptamers Nucleotide 021001 nanoscience & nanotechnology Silicon Dioxide Amperometry 0104 chemical sciences Carcinoembryonic Antigen biology.protein Nanoparticles Gold 0210 nano-technology Biosensor |
| Zdroj: | Analytica chimica acta. 1061 |
| ISSN: | 1873-4324 |
| Popis: | We report herein the design of a novel biosensing strategy for the detection of carcinoembryonic antigen (CEA), based on the use of Janus-type nanoparticles having Au and silica opposite faces as integrated electrochemical biorecognition-signaling system. The Janus nanoparticles were properly functionalized with horseradish peroxidase on the silica surface to act as signaling element, and a biotin thiol-modified anti-CEA DNA hairpin aptamer the Au face to assemble the biorecognition element. The sensing approach relies on the first specific recognition of CEA by the bifunctionalized Janus nanoparticles, causing unfolding of the DNA hairpin structure and unmasking the biotin residues at the aptamer chain. This CEA-Janus nanoparticle complex was then captured by avidin-modified Fe3O4@SiO2 NanoCaptors®, allowing further magnetic deposition on carbon screen printed electrodes for the amperometric detection of the cancer biomarker. The Janus nanoparticles-based aptasensor was able to detect CEA in the range from 1 to 5000 ng mL−1 (5.5 pM–28 nM) with a detection limit of 210 pg mL−1 (1.2 pM). The aptasensor also showed high reproducibility and storage stability, and was successfully validated in human serum. |
| Databáze: | OpenAIRE |
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