Interferon regulatory transcription factors are constitutively expressed and spatially regulated in the mouse lens
| Autor: | Charles E. Egwuagu, Bassey J.S. Efiok, Ana B. Chepelinsky, Wenmei Li, Chandrasekharam N. Nagineni |
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| Rok vydání: | 1999 |
| Předmět: |
Genetically modified mouse
Mice Transgenic ICSBP Cell Line Interferon-gamma Mice STAT1 Interferon Lens Crystalline medicine Animals RNA Messenger Transcription factor Molecular Biology LSIRF/Pip IRF-2 Regulation of gene expression IRF-1 Mice Inbred BALB C biology Gene Expression Regulation Developmental Nuclear Proteins Cell Biology Interferon-Stimulated Gene Factor 3 Phosphoproteins Molecular biology Immunohistochemistry Lens Fiber DNA-Binding Proteins Repressor Proteins medicine.anatomical_structure Lens (anatomy) Interferon Regulatory Factors lens differentiation biology.protein Interferon Regulatory Factor-2 IFNγ medicine.drug Interferon regulatory factors Developmental Biology Interferon Regulatory Factor-1 Signal Transduction Transcription Factors |
| Zdroj: | Developmental biology. 210(1) |
| ISSN: | 0012-1606 |
| Popis: | Interferon regulatory factors (IRFs) are a family of transcription factors involved in regulation of cell growth and immunological responses. Nine IRFs have been described and they are expressed in a variety of cells, except for ICSBP and LSIRF/Pip, which are thought to be expressed exclusively in immune cells. Here, we show that IRF-1, IRF-2, ICSBP, and LSIRF/Pip are constitutively expressed in the mouse lens. These IRFs are present in both the cytoplasm and the nuclei of lens cells. However, the nuclear and cytoplasmic proteins exhibit distinct mobilities on SDS/PAGE. We further show that in the developing mouse lens, IRF-1 and IRF-2 are expressed at high levels in differentiated lens fiber cells with very low and barely detectable levels in undifferentiated lens epithelial cells. Although the level of ICSBP expression is very low in the normal mouse lens, in transgenic mice with constitutive expression of interferon γ in the lens, its level is markedly elevated and ICSBP expression is detected exclusively in the nuclei of undifferentiated lens cells. Taken together, our data suggest that expression of IRF transcription factors is spatially regulated in the lens and that distinct IRFs may contribute to differential gene regulation in the epithelial and fiber compartments of the vertebrate lens. |
| Databáze: | OpenAIRE |
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