Actin stabilization induces apoptosis in cultured porcine epithelial cell rests of Malassez

Autor: Takashi Muramatsu, F. Kobayashi, Masataka Suehara, Natsuko Aida, Ryo Sako, T. Ushikubo, Masahiro Furusawa
Rok vydání: 2015
Předmět:
Zdroj: International Endodontic Journal. 49:663-669
ISSN: 0143-2885
DOI: 10.1111/iej.12494
Popis: Aim To test whether actin stabilization by jasplakinolide induces inhibition of cell viability and apoptosis in epithelial cell rests of Malassez (ERM). Methodology ERM derived from porcine were spread in a 96-well dish (5 × 104/well) using Dulbecco's modified Eagle's medium. The actin-specific stabilization reagent, jasplakinolide, was incorporated into the culture medium and incubated for 24 h. To evaluate cell viability, the WST-1 assay was carried out and absorption (450 nm) was measured. To detect apoptotic cells, monoclonal antibody to single-strand DNA (ssDNA) was used and absorption (405 nm) was measured. Actin stabilization and apoptosis induced by jasplakinolide were morphologically investigated by staining with Alexa Fluor 568 phalloidin and observed under a fluorescent microscope. As a negative control, DMSO was used instead of jasplakinolide. Differences between the jasplakinolide-treated group and the control group were analysed statistically using the Student's t-test. Results Cell viability decreased in a concentration-dependent manner, and cell viability in the jasplakinolide-treated ERM was lower than that in nontreated ERM (n = 16, P
Databáze: OpenAIRE
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