Application of vacA Sequencing in Helicobacter pylori for Classification of Specimens from Healthy Persons, and from Hepatobiliary and Gastroduodenal Patients
| Autor: | Kookwan Sawadpanich, Narong Khuntikeo, Chariya Chomvarin, Wongwarut Boonyanugomol, Phattharaphon Wongphutorn, Kittipan Samerpitak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty biology business.industry Helicobacter pylori biology.organism_classification bacterial infections and mycoses h. pylori Applied Microbiology and Biotechnology Gastroenterology Microbiology digestive system diseases QR1-502 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Internal medicine gastrointestinal diseases medicine vacuolating cytotoxin a gene hepatobiliary diseases and healthy persons business Biotechnology |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 11, Iss 1, Pp 01-07 (2017) |
| ISSN: | 0973-7510 |
| Popis: | Helicobacter pylori is an important causative agent of gastrointestinal and hepatobiliary diseases. The aim of this study was to investigate phylogenetic relationships among H. pylori strains from the oral cavities of healthy individuals, gastric biopsies from gastroduodenal (GI) patients and bile samples from hepatobiliary (HB) patients in the northeast of Thailand. The DNA sequences of a portion of the vacuolating cytotoxin A gene (vacA) were investigated. Phylogenetic trees were constructed using the neighbor-joining and maximum-likelihood methods. The vacA sequences of H. pylori fell into four main groups on the trees. Strains from healthy persons fell into two widely separated but well-supported groups, while most H. pylori vacA sequences from HB patients were distributed in another two groups. In contrast, the H. pylori strains from GI patients were scattered across the tree, without a clear geographical pattern. In conclusion, the sequence of vacA may be useful to classify the genetic relationship of H. pylori derived from different sources. |
| Databáze: | OpenAIRE |
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