Expression of human heme oxygenase-1 in the thick ascending limb attenuates angiotensin II–mediated increase in oxidative injury11See Editorial by Chang and Perrella, p. 1968
| Autor: | Sylvia Shnouda, Michal L. Schwartzman, Shuo Quan, Alberto Nasjletti, Nader G. Abraham, Liming Yang, Alvin I. Goodman |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Na+-K+-Cl− cotransporter
biology oxidative injury adenovirus heme oxygenase angiotensin II TALH medicine.disease_cause Molecular biology Angiotensin II Oxygen tension Heme oxygenase cyclooxygenase chemistry.chemical_compound chemistry Biochemistry Downregulation and upregulation Apoptosis Nephrology Na-K-Cl cotransporter biology.protein medicine Heme Oxidative stress |
| Zdroj: | Kidney International. 65(5):1628-1639 |
| ISSN: | 0085-2538 |
| DOI: | 10.1111/j.1523-1755.2004.00562.x |
| Popis: | Expression of human heme oxygenase-1 in the thick ascending limb attenuates angiotensin II–mediated increase in oxidative injury. Background Heme oxygenase-1 (HO-1) catalyzes the conversion of heme to bilirubin, carbon monoxide (CO), and free iron, thus controlling the level of cellular heme. The medullary thick ascending limb of the loop of Henle (TALH) is situated in a site of markedly diminished oxygen tension and, as such, is highly vulnerable to ischemic insult. We hypothesize that selective upregulation of HO-1 in TALH by gene transfer attenuates oxidative stress caused by angiotensin II (Ang II). Methods An adenoviral vector expressing the human HO-1 under the control of the TALH-specific promoter [Na + -K + -Cl − cotransporter (NKCC2 promoter)] was constructed and the cell specific expression of the recombinant adenovirus was examined using several types of cells, including endothelial, vascular smooth muscle, and TALH cells. The effects of HO-1 transduction on HO-1 expression, HO activity and the response to Ang II with respect to cyclooxygenase-2 (COX-2) up-regulation and oxidative injury [growth-stimulating hormone (GSH) levels and cell death] were determined. Results Western blot and reverse transcription-polymerase chain reaction (RT-PCR) revealed that human HO-1 was selectively expressed in primary cultured TALH cells following infection with Ad-NKCC2-HO-1. In TALH cells infected with Ad-NKCC2-HO-1, Ang II-stimulated prostaglandin E 2 (PGE 2 ) levels were reduced by 40%. Ang II caused a marked decrease in GSH levels and this decrease was greatly attenuated in TALH cells transduced with Ad-NKCC2-HO-1. Moreover, Ang II–mediated DNA degradation was completely blocked by the site-specific expression of human HO-1 gene. Conclusion These results indicate that TALH cell survival after exposure to oxidative stress injury may be facilitated by selective upregulation of HO-1, thusly blocking inflammation and apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|