Conformational studies of d(m5CpGpm5CpG) and d(CpGpCpG) by 1H and 31P NMR
| Autor: | Muriel Delepierre, Bernard P. Roques, J. Igolen, Béatrce Langlois D'estaintot |
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| Rok vydání: | 1986 |
| Předmět: |
Coupling constant
Steric effects Magnetic Resonance Spectroscopy Proton Chemistry Stereochemistry Chemical shift Temperature Phosphorus Stereoisomerism Nuclear Overhauser effect Biochemistry Methylation Homonuclear molecule chemistry.chemical_compound Heteronuclear molecule Oligodeoxyribonucleotides Nucleic Acid Conformation Protons DNA |
| Zdroj: | European journal of biochemistry. 161(3) |
| ISSN: | 0014-2956 |
| Popis: | Exhaustive conformational studies of d(CpG)2 and d(m5CpG)2, two convenient targets for DNA bisintercalating drugs, have been carried out by 1H and 31P NMR in low salt concentration and in the presence of 30% ethanol. Unambiguous 31P assignments of the B for are obtained with low-power heteronuclear decoupling experiments, while 31P assignments in the Z form are obtained by two-dimensional homonuclear chemical exchange experiments. The 31P chemical shifts and 3JH3'P coupling constants studied at various temperatures in methylated and non-methylated tetranucleotides, are interpreted as resulting from conformational differences between the compounds. These features are corroborated by homonuclear proton nuclear Overhauser effect experiments showing the steric role of the 5-methylcytosine in the induction of an alternating B form in d(m5CpG)2. |
| Databáze: | OpenAIRE |
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