Physicochemical properties and cytocompatibility assessment of non-degradable scaffolds for bone tissue engineering applications
| Autor: | Rui L. Reis, Filipe Samuel Silva, H. Pereira, F. Bartolomeu, Fátima Raquel Maia, J. M. Oliveira, Ibrahim Fatih Cengiz |
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| Rok vydání: | 2020 |
| Předmět: |
Bone Banks
Bone Regeneration Materials science Biomedical Engineering 02 engineering and technology Bone tissue Bone and Bones Biomaterials 03 medical and health sciences 0302 clinical medicine Elastic Modulus Peek medicine Humans Saos-2 cells Elastic modulus Cell Proliferation Tissue Engineering Tissue Scaffolds Regeneration (biology) 030206 dentistry Stress shielding 021001 nanoscience & nanotechnology medicine.disease medicine.anatomical_structure Mechanics of Materials Osteosarcoma 0210 nano-technology Porosity Biomedical engineering |
| Zdroj: | Journal of the Mechanical Behavior of Biomedical Materials. 112:103997 |
| ISSN: | 1751-6161 |
| DOI: | 10.1016/j.jmbbm.2020.103997 |
| Popis: | Bone is a dynamic tissue with an amazing but yet limited capacity of self-healing. Bone is the second most transplanted tissue in the world and there is a huge need for bone grafts and substitutes which lead to a decrease in bone banks donors. In this study, we developed three-dimensional scaffolds based on Ti6Al4V, ZrO2 and PEEK targeting bone tissue engineering applications. Experimental mechanical compressive tests and finite element analyses were carried out to study the mechanical performance of the scaffolds. Overall, the scaffolds presented different hydrophilicity properties and a reduced elastic modulus when compared with the corresponding solid materials which can in some extension minimize the phenomenon of stress shielding. The ability as a scaffold material for bone tissue regeneration applications was evaluated in vitro by seeding human osteosarcoma (SaOS-2) cells onto the scaffolds. Then, the successful culture of SaOS-2 cells on developed scaffolds was monitored by assessment of cell's viability, proliferation and alkaline phosphatase (ALP) activity up to 14 days of culturing. The in vitro results revealed that Ti6Al4V, ZrO2 and PEEK scaffolds were cytocompatible allowing the successful culture of an osteoblastic cell line, suggesting their potential application in bone tissue engineering. Statement of Significance. The work presented is timely and relevant since it gathers both the mechanical and cellular study of non-degradable cellular structures with the potential to be used as bone scaffolds. This work allow to investigate three possible bone scaffolds solutions which exhibit a significantly reduced elastic modulus when compared with conventional solid materials. While it is generally accepted that the Ti6Al4V, ZrO2 and PEEK are candidates for such applications a further study of their features and their comparison is extremely important for a better understanding of their potential. |
| Databáze: | OpenAIRE |
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