VEGF and BMP-6 enhance bone formation mediated by cloned mouse osteoprogenitor cells
| Autor: | Quanjun Cui, Xiuli Wang, Abhijit S. Dighe, Xian-xi Liu, Fuai Cui, Gary Balian |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Bone Regeneration animal structures Bone Morphogenetic Protein 6 Angiogenesis Osteocalcin Clinical Biochemistry Bone morphogenetic protein 8A Cell Gene Expression Core Binding Factor Alpha 1 Subunit Transfection Mice chemistry.chemical_compound Calcification Physiologic Endocrinology Osteogenesis In vivo Internal medicine medicine Animals Humans Cells Cultured biology Mesenchymal Stem Cells Genetic Therapy Cell Biology Alkaline Phosphatase Cell biology RUNX2 Vascular endothelial growth factor medicine.anatomical_structure chemistry embryonic structures biology.protein Alkaline phosphatase |
| Zdroj: | Growth Factors. 28:306-317 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977194.2010.484423 |
| Popis: | New strategies such as combined utilization of growth factors may provide a better treatment for difficult fractures. We have demonstrated enhanced angiogenesis and osteogenesis through the actions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-6 (BMP-6) on the osteogenic differentiation of a cloned mouse osteoprogenitor cell in vitro and ectopic bone formation in vivo. Human VEGF and BMP-6 genes expressed together produced a significant increase in alkaline phosphatase activity, expression of the RunX2 and osteocalcin genes and mineralization. Microcomputed tomographic analysis of subcutaneous implants consisting of cells transfected with VEGF and BMP-6 cDNA and delivered on a 3D poly (lactic-co-glycolic acid) scaffold confirmed the additive effects between VEGF and BMP-6. Ectopic bone formation in the VEGF plus BMP-6 group was greatest compared to that in either VEGF or BMP-6 alone. This is the first study that demonstrates osteogenesis in vitro and in vivo through the additive effects of VEGF and BMP-6. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |