Relationship between SIRT1 gene expression level and disease in age-related cataract cases
| Autor: | Müjgan Özdemir Erdoğan, Mustafa Solak, Saliha Handan Yildiz, Cem Karaosmanoğlu, Nuray Varol, Nihat Buğra Ağaoğlu, Rahmi Duman |
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| Přispěvatelé: | Varol, Nuray, Yıldız, Saliha Handan, Karaosmanoğlu, Cem, Özdemir Erdoğan, Müjgan, Solak, Mustafa |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male genetic structures 030204 cardiovascular system & hematology Bioinformatics Age-related cataract epigenetics mechanisms SIRT1 Polymerase Chain Reaction Article Cataract Epigenesis Genetic 03 medical and health sciences SIRT1 0302 clinical medicine Sirtuin 1 Cataracts medicine Humans Genetic Predisposition to Disease Age-related cataract Prospective Studies Epigenetics Cells Cultured Cerrahi Aged Aged 80 and over 0303 health sciences biology 030306 microbiology business.industry General Medicine Middle Aged medicine.disease eye diseases epigenetics mechanisms Real-time polymerase chain reaction Sirtuin biology.protein Anterior Capsule of the Lens Biomarker (medicine) Female Histone deacetylase business |
| Zdroj: | Turkish Journal of Medical Sciences Volume: 49, Issue: 4 1068-1072 |
| ISSN: | 1300-0144 1303-6165 |
| Popis: | Background/aim: Age-related cataract is the most important visual impairment all over the world. Epigenetic modifications, especially overexpression of histone deacetylases, have become the focus of interest for cataract development in recent years. Sirtuin 1 (SIRT1), a class II histone deacetylase and a member of the sirtuin family, is one of the best-characterized histone deacetylases and has a pivotal role in age-related diseases. However, the association of SIRT1 with age-related cataracts has not yet been fully elucidated. Therefore, we aimed to determine the expression of SIRT1 in age-related cataract patients.Materials and methods: Expressions of SIRT1 were evaluated by quantitative polymerase chain reaction (qPCR) in patients and healthy controls. RNA samples were collected from the anterior capsule and peripheral blood samples of age-related cataract patients. Human lens epithelial cell line B3 and peripheral blood samples of healthy subjects were used as controls. Results: We determined that the expression of SIRT1 in blood and anterior capsule samples increased significantly compared to the control group (P < 0.05). Conclusion: The expression level of SIRT1 plays a vital role in the development of age-related cataract and it can be used as a biomarker. Thus, SIRT1 inhibitors can be used in the treatment of age-related cataract disease. |
| Databáze: | OpenAIRE |
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