Cell-Instructive Surface Gradients of Photoresponsive Amyloid-like Fibrils
| Autor: | Kübra Kaygisiz, Tanja Weil, Franz J. Mayer, Hans Joachim Räder, Christopher V. Synatschke, Adriana Maria Ender |
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| Rok vydání: | 2021 |
| Předmět: |
Amyloid
peptide amyloid fiber Cell Biomedical Engineering Peptide Fibril Article Epitope Biomaterials Cell Movement photocontrolled cell adhesion Cell Adhesion medicine Humans MALDI-MSI Cell adhesion chemistry.chemical_classification Chemistry bioactive gradient Cell migration Adhesion medicine.anatomical_structure A549 Cells Biophysics Peptides Oligopeptides Linker |
| Zdroj: | ACS Biomaterials Science & Engineering |
| ISSN: | 2373-9878 |
| Popis: | Gradients of bioactive molecules play a crucial role in various biological processes like vascularization, tissue regeneration, or cell migration. To study these complex biological systems, it is necessary to control the concentration of bioactive molecules on their substrates. Here, we created a photochemical strategy to generate gradients using amyloid-like fibrils as scaffolds functionalized with a model epitope, that is, the integrin-binding peptide RGD, to modulate cell adhesion. The self-assembling β-sheet forming peptide (CKFKFQF) was connected to the RGD epitope via a photosensitive nitrobenzyl linker and assembled into photoresponsive nanofibrils. The fibrils were spray-coated on glass substrates and macroscopic gradients were generated by UV-light over a centimeter-scale. We confirmed the gradient formation using matrix-assisted laser desorption ionization mass spectroscopy imaging (MALDI-MSI), which directly visualizes the molecular species on the surface. The RGD gradient was used to instruct cells. In consequence, A549 adapted their adhesion properties in dependence of the RGD-epitope density. |
| Databáze: | OpenAIRE |
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