Rearrangement of the rheumatoid factor-related germline gene Vg and bcl-2 expression in lymphoproliferative disorders in patients with Sjögren's syndrome
| Autor: | Ichiro Saito, Susumu Sugai, S Takeshita, Shiro Shimizu, Masaki Y, Nobuyuki Miyasaka, Junko Tachibana |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Lymphoepithelial lesion Immunology Immunoglobulin Variable Region Lymphoproliferative disorders Gene Expression Biology Cross Reactions Lymphocyte Activation Salivary Glands Pathology and Forensic Medicine Germline mutation Rheumatoid Factor Proto-Oncogene Proteins medicine Pseudolymphoma Immunology and Allergy Rheumatoid factor Humans Aged Aged 80 and over Gene Rearrangement B-Lymphocytes Genes Immunoglobulin Antibodies Monoclonal Gene rearrangement DNA Middle Aged medicine.disease Lymphoproliferative Disorders Sjogren's Syndrome Proto-Oncogene Proteins c-bcl-2 Monoclonal biology.protein Cancer research Female Antibody Cell Division |
| Zdroj: | Clinical immunology and immunopathology. 72(2) |
| ISSN: | 0090-1229 |
| Popis: | Of 250 patients with Sjogren's syndrome (SS) (190 with 1 degree SS and 60 with 2 degrees SS), 60 patients demonstrated lymphoproliferative disorders (LPD): 41 patients had monoclonal gammopathy (MG), 4 had pseudolymphoma, 3 had in situ monoclonal lymphoproliferation, and 12 had malignant lymphoma. Rearrangement of the rheumatoid factor (RF)-related germline gene Vg (associated with the monoclonal RF SF18/2) was demonstrated in 24 of 50 genomic DNAs from the peripheral blood leukocytes of SS patients. It was found in none of 20 DNAs from normal subjects. This suggests an almost 50% incidence of a germline Ig gene rearrangement in SS patients without clinical MG. Lymphocytes composing the lymphoepithelial lesion (LEL) in the major salivary glands of SS patients expressed the oncogene bcl-2 protein in 4 out of 6 patients. The progression of SS from benign to malignant lymphoproliferation may be related to suppression of apoptotic death by bcl-2. These findings suggest that (i) RF clones are activated in SS with little or no somatic mutation resulting in monoclonal proliferation and (ii) the LEL in the salivary gland is one site for monoclonal B cell proliferation and emerging malignant lymphoma. |
| Databáze: | OpenAIRE |
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