Lineage tracing using matrilin-1 gene expression reveals that articular chondrocytes exist as the joint interzone forms
| Autor: | Sharon L. Dover, Attila Aszódi, Gillian A. Wallis, Raymond P. Boot-Handford, Gareth Hyde |
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| Rok vydání: | 2006 |
| Předmět: |
musculoskeletal diseases
Cartilage Articular Cellular differentiation Cre recombinase Mice Transgenic Biology Knee Joint Lineage tracing Article Extracellular matrix 03 medical and health sciences Exon Mice 0302 clinical medicine Chondrocytes Collagen II Gene expression medicine Animals Matrilin Proteins Cell Lineage Joint development Knee development Molecular Biology 030304 developmental biology Glycoproteins 0303 health sciences Extracellular Matrix Proteins Cartilage Synovial Membrane Cell Differentiation Anatomy Cell Biology Exons musculoskeletal system Cell biology medicine.anatomical_structure Animals Newborn embryonic structures Matrilin-1 Articular chondrocytes Joints Synovial membrane 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental biology. 304(2) |
| ISSN: | 0012-1606 |
| Popis: | We have developed a mouse in which the Cre recombinase gene has been targeted to exon 1 of the matrilin-1 gene (Matn1) to investigate the origins of articular chondrocytes and the development of the knee joint. Analysis of joints from offspring of Matn1-Cre/R26R crosses demonstrated that articular chondrocytes are derived from cells that have never expressed matrilin-1 whereas the remainder of the chondrocytes in the cartilage anlagen expresses matrilin-1. A band of chondrocytes adjacent to the developing interzone in the E13.5 day knee joint became apparent because these chondrocytes did not turn on expression of matrilin-1 in contrast to the other chondrocytes of the anlagen. The chondrocytes of the presumptive articular surface therefore appear to arise directly from a subpopulation of early chondrocytes that do not activate matrilin-1 expression rather than by redifferentiation from the flattened cells of the interzone. In addition, lineage tracing using both Matn1-Cre/R26R and Col2a1-Cre/R26R lines indicated that non-cartilaginous structures in the knee such as cruciate ligament, synovium and some blood vessels are formed by cells derived from the early chondrocytes of the anlagen. |
| Databáze: | OpenAIRE |
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