Cervical Squamous Intraepithelial Lesions Are Associated with Differences in the Vaginal Microbiota of Mexican Women

Autor: Patricia Morán, B. B. Finlay, C. Ximenez-Garcia, Marie-Claire Arrieta, Horacio Pérez-Juárez, Angélica Serrano-Vázquez, M. E. Perez-Rodriguez, M. E. Nieves-Ramírez, Oswaldo Partida-Rodriguez
Rok vydání: 2021
Předmět:
Physiology
Social Determinants of Health
Uterine Cervical Neoplasms
Alphapapillomavirus
Cervical carcinogenesis
0302 clinical medicine
RNA
Ribosomal
16S
Brevibacterium
Cervical cancer
0303 health sciences
education.field_of_study
Ecology
biology
Microbiota
HPV infection
virus diseases
QR1-502
female genital diseases and pregnancy complications
3. Good health
Actinobacteria
Infectious Diseases
030220 oncology & carcinogenesis
Vagina
Vaginal microbiome
Female
Research Article
Microbiology (medical)
Female circumcision
Adult
HPV
Sociodemographic Factors
Population
Microbiology
03 medical and health sciences
Genetics
medicine
Lactobacillus iners
Humans
education
Mexico
030304 developmental biology
vaginal microbiota
General Immunology and Microbiology
Bacteria
business.industry
HPV Positive
Papillomavirus Infections
squamous intraepithelial lesions
Epithelial Cells
Cell Biology
medicine.disease
biology.organism_classification
Uterine Cervical Dysplasia
Lactobacillus
Immunology
Dysbiosis
16S rRNA gene
business
Zdroj: Microbiology Spectrum
Microbiology Spectrum, Vol 9, Iss 2 (2021)
ISSN: 2165-0497
Popis: Cervical cancer is an important health concern worldwide and is one of the leading causes of death in Mexican women. Previous studies have shown changes in the female genital tract microbe community related to human papillomavirus (HPV) infection and cervical cancer; yet, this link remains unexplored in many human populations. This study evaluated the vaginal bacterial community among Mexican women with precancerous squamous intraepithelial lesions (SIL). We sequenced the V3 region of the 16S rRNA gene in cervical samples from 228 Mexican women, including 121 participants with SIL, most of which were HPV positive, and 107 healthy women without HPV infection or SIL. The presence of SIL was associated with changes in composition (beta diversity) and with a higher species richness (Chao1). A comparison of HPV-positive women with and without SIL showed that microbiota changes occurred even in the absence of SIL. Multivariate association with linear models (MaAsLin) analysis yielded independent associations between HPV infection and an increase in the relative abundance of Brachybacterium conglomeratum and Brevibacterium aureum as well as a decrease in two Lactobacillus iners operational taxonomic units (OTUs). We also identified a positive independent association between HPV-16, the most common HPV subtype linked to SIL, and Brachybacterium conglomeratum. Our work indicates that HPV infection leading to SIL is primarily associated with shifts in vaginal microbiota composition, some of which may be specific to this human population. IMPORTANCE Human papillomavirus (HPV) plays a critical role in cervical carcinogenesis but is not sufficient for cervical cancer development, indicating the involvement of other factors. The vaginal microbiota is an important factor in controlling infections caused by HPV, and, depending on its composition, it can modulate the microenvironment in vaginal mucosa against viral infections. Ethnic and sociodemographic factors influence differences in vaginal microbiome composition, which underlies the dysbiotic patterns linked to HPV infection and cervical cancer across different populations of women. Here, we provide evidence for associations between vaginal microbiota patterns and HPV infection linked to ethnic and sociodemographic factors. To our knowledge, this is the first report of the species Brevibacterium aureum and Brachybacterium conglomeratum linked to HPV infection or squamous intraepithelial lesions (SIL).
Databáze: OpenAIRE
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