Vitamin C prevents epidermal damage induced by PM‐associated pollutants and UVA1 combined exposure
| Autor: | Jeremie Soeur, Martine Zanini, Laurent Marrot, Samia Boudah, Hélène Zucchi, Joaquim Lima, Ariane Dimitrov |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Ultraviolet Rays Ascorbic Acid Dermatology Skin Diseases Biochemistry 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ultrafine particle Humans Polycyclic Aromatic Hydrocarbons Molecular Biology Pollutant integumentary system Vitamin C Epidermis (botany) Chemistry Vitamins Molecular biology 030104 developmental biology Loricrin Pyrene Particulate Matter Epidermis Phototoxicity Filaggrin |
| Zdroj: | Experimental Dermatology. 30:1693-1698 |
| ISSN: | 1600-0625 0906-6705 |
| Popis: | Particulate matter is suspected to be substantially involved in pollution-induced health concerns. In fact, ultrafine particles (UFPs) contain polycyclic aromatic hydrocarbons (PAHs) known as mutagenic, cytotoxic and sometimes phototoxic. Since UFPs reach blood circulation from lung alveoli, deep skin is very likely contaminated by PAHs coming from either skin surface or blood. As photoreactive, benzo(a)pyrene (BaP) or indenopyrene (IcdP) is involved in the interplay between pollution and sunlight. In order to better characterize this process, experiments were carried out on reconstructed human epidermis (RHE) in a protocol mimicking realistic exposure. Concentrations of PAHs comparable to those generally reported in blood were used together with chronic irradiation to low dose UVA1. On a histological level, damaged cells mainly accumulated in a suprabasal situation, thus reducing living epidermis thickness. Stress markers such as IL1-α or MMP3 secretion increased, and surprisingly, the histological position of Transglutaminase-1 within epidermis was disturbed, whereas position of other differentiation markers (keratin-10, filaggrin, loricrin) remained unchanged. When vitamin C was added in culture medium, a very significant protection involving all markers was noticed. In conclusion, we provide here a model of interest to understand the epidermal deleterious consequences of pollution and to select efficient protective compounds. |
| Databáze: | OpenAIRE |
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