Placenta-derived macaque trophoblast stem cells: differentiation to syncytiotrophoblasts and extravillous trophoblasts reveals phenotypic reprogramming
Autor: | Michael G. Meyer, Logan T Keding, Gregory J. Wiepz, Thaddeus G. Golos, Katherine D Mean, Kamryn M. Kroner, Jenna Kropp Schmidt, Michelle R Koenig, Mario J. Bertogliat, Lindsey N. Block, Brittany M. Dusek, Avery R Kallio |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Placenta Population lcsh:Medicine Syncytiotrophoblasts Macaque Article 03 medical and health sciences 0302 clinical medicine Syncytiotrophoblast Pregnancy biology.animal Rhesus macaque medicine Animals education lcsh:Science Cell Proliferation education.field_of_study 030219 obstetrics & reproductive medicine Multidisciplinary biology Stem Cells lcsh:R Trophoblast Placentation Cell Differentiation Trophoblasts Gonadotropin secretion Cell biology Phenotype 030104 developmental biology medicine.anatomical_structure embryonic structures Macaca Female lcsh:Q Cytotrophoblasts |
Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-22 (2020) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Nonhuman primates are excellent models for studying human placentation as experimental manipulations in vitro can be translated to in vivo pregnancy. Our objective was to develop macaque trophoblast stem cells (TSCs) as an in vitro platform for future assessment of primate trophoblast development and function. Macaque TSC lines were generated by isolating first and second trimester placental villous cytotrophoblasts followed by culture in TSC medium to maintain cellular proliferation. TSCs grew as mononuclear colonies, whereas upon induction of syncytiotrophoblast (ST) differentiation multinuclear structures appeared, indicative of syncytium formation. Chorionic gonadotropin secretion was > 4000-fold higher in ST culture media compared to TSC media. The secretion of chorionic gonadotropin by TSC-derived ST reflects a reprogramming of macaque TSCs to an earlier pregnancy phenotype. Characteristic trophoblast hallmarks were defined in TSCs and ST including expression of C19MC miRNAs and the macaque placental nonclassical MHC class I molecule, Mamu-AG. Extravillous trophoblasts (EVTs) were derived that express macaque EVT markers Mamu-AG and CD56, and also secrete high levels of MMP2. Our analyses of macaque TSCs suggests that these cells represent a proliferative, self-renewing population capable of differentiating to STs and EVTs in vitro thereby establishing an experimental model of primate placentation. |
Databáze: | OpenAIRE |
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