Production and characteristics of a novel chicken egg yolk antibody (IgY) against periodontitis-associated pathogens
| Autor: | Lu Minglei, Linjin Yu, He Pan, Suqing Zhao, Huiyi Yang, Chenggang Jia, Xiangguang Li, Xiuting Wei, Qiyi He |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) agglutination food.ingredient Aggregatibacter Infectious and parasitic diseases RC109-216 Microbiology biofilm 03 medical and health sciences 0302 clinical medicine food porphyromonas gingivalis Yolk egg yolk antibody medicine Dentistry (miscellaneous) periodontitis Porphyromonas gingivalis Periodontitis aggregatibacter actinomycetemcomitans biology Biofilm Aggregatibacter actinomycetemcomitans 030206 dentistry biology.organism_classification medicine.disease QR1-502 stomatognathic diseases Agglutination (biology) 030104 developmental biology Infectious Diseases biology.protein Research-Article Original Article Antibody |
| Zdroj: | Journal of Oral Microbiology, Vol 12, Iss 1 (2020) Journal of Oral Microbiology article-version (VoR) Version of Record |
| ISSN: | 2000-2297 |
| Popis: | Periodontitis is a bacterial biofilm-induced oral disease, mostly caused by Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis). Oral administration of chicken egg yolk antibody (IgY) is a promising nutritional strategy to control pathogen infections. The objective of this study was to produce an A. actinomycetemcomitans- and P. gingivalis-specific IgY and evaluate its effects on bacterial agglutination and biofilm formation. Thirty laying hens were immunized with a complex of lysate containing typical molecular weights of membrane proteins of A. actinomycetemcomitans and P. gingivalis. IgY was isolated by polyethylene glycol 6000 and ammonium sulfate and purified by dialysis. The results of enzyme-linked immunosorbent assay showed that the obtained IgY were specific to both A. actinomycetemcomitans and P. gingivalis. In addition, immunoelectron microscopy scanning and crystal violet staining showed that the IgY could bind to cell wall of the pathogens and efficiently accelerate agglutination and inhibit biofilm formation. Furthermore, the activity of the IgY remained stable at different temperature, pH, and storage period. This is the first report that a novel two-in-one IgY was produced to modulate the agglutination and biofilm formation of A. actinomycetemcomitans and P. gingivalis, suggesting the potential of IgY to control periodontitis caused by oral pathogens. |
| Databáze: | OpenAIRE |
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