Analyses of Transcriptomics Cell Signalling for Pre-Screening Applications in the Integrated Approach for Testing and Assessment of Non-Genotoxic Carcinogens

Autor:	Yusuke Oku, Federica Madia, Pierre Lau, Martin Paparella, Timothy McGovern, Mirjam Luijten, Miriam N. Jacobs
Rok vydání:	2022
Předmět:	organisation for economic co-operation and development (OECD) Carcinogenesis Carcinogenicity Tests Organic Chemistry General Medicine Catalysis Computer Science Applications Inorganic Chemistry integrated approaches to testing and assessment (IATA) transcriptomics carcinogenicity gene expression Carcinogens Humans non-genotoxic carcinogen(s) (NGTxC(s)) Biological Assay Physical and Theoretical Chemistry Transcriptome Molecular Biology Spectroscopy
Zdroj:	International journal of molecular sciences. 23(21)
ISSN:	1422-0067
Popis:	With recent rapid advancement of methodological tools, mechanistic understanding of biological processes leading to carcinogenesis is expanding. New approach methodologies such as transcriptomics can inform on non-genotoxic mechanisms of chemical carcinogens and can be developed for regulatory applications. The Organisation for the Economic Cooperation and Development (OECD) expert group developing an Integrated Approach to the Testing and Assessment (IATA) of Non-Genotoxic Carcinogens (NGTxC) is reviewing the possible assays to be integrated therein. In this context, we review the application of transcriptomics approaches suitable for pre-screening gene expression changes associated with phenotypic alterations that underlie the carcinogenic processes for subsequent prioritisation of downstream test methods appropriate to specific key events of non-genotoxic carcinogenesis. Using case studies, we evaluate the potential of gene expression analyses especially in relation to breast cancer, to identify the most relevant approaches that could be utilised as (pre-) screening tools, for example Gene Set Enrichment Analysis (GSEA). We also consider how to address the challenges to integrate gene panels and transcriptomic assays into the IATA, highlighting the pivotal omics markers identified for assay measurement in the IATA key events of inflammation, immune response, mitogenic signalling and cell injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d33a2aab32d6d0eb4c980f73b3e184c https://pubmed.ncbi.nlm.nih.gov/36361516 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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