Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity
| Autor: | Grazziela P. Figueredo, Judith Olzhausen, Xiaowei Wang, Eliane Isabelle Marti, Claudio Rhyner, Nuzul Noorahya Jambari, Marcos J. C. Alcocer, José Javier Pérez-Barea |
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| Přispěvatelé: | University of Zurich, Marti, E |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Allergy Microarray 3400 General Veterinary Immunology Protein Array Analysis 610 Medicine & health Disease Biology medicine.disease_cause Ceratopogonidae Allergen 10183 Swiss Institute of Allergy and Asthma Research medicine Hypersensitivity Animals Diagnosis Computer-Assisted Horses INSECT BITE HYPERSENSITIVITY Skin 2403 Immunology General Veterinary Models Immunological A protein Insect Bites and Stings Mathematical Concepts Allergens Immunoglobulin E medicine.disease In vitro Case-Control Studies Protein microarray Female Horse Diseases |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known. |
| Databáze: | OpenAIRE |
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