Direct anti-inflammatory effects of granulocyte colony-stimulating factor (G-CSF) on activation and functional properties of human T cell subpopulations in vitro
| Autor: | Galina V. Seledtsova, Viacheslav Anatolievich Shmarov, V. V. Malashchenko, O. B. Melashchenko, Maxsim Evgenievich Meniailo, N. D. Gazatova, Victor I. Seledtsov, Andrei G. Goncharov |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine Naive T cell T-Lymphocytes medicine.medical_treatment T cell CD3 Immunology Anti-Inflammatory Agents Biology CD38 Lymphocyte Activation Interferon-gamma 03 medical and health sciences CD28 Antigens T-Lymphocyte Subsets Granulocyte Colony-Stimulating Factor medicine Humans IL-2 receptor Interleukin-2 Receptor alpha Subunit CD28 Macrophage Activation Acquired immune system Molecular biology Healthy Volunteers 030104 developmental biology Cytokine medicine.anatomical_structure biology.protein Cytokines Female Immunologic Memory |
| Zdroj: | Cellular Immunology. 325:23-32 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/j.cellimm.2018.01.007 |
| Popis: | We investigated the direct effects of human granulocyte colony-stimulating factor (G-CSF) on functionality of human T-cell subsets. CD3+ T-lymphocytes were isolated from blood of healthy donors by positive magnetic separation. T cell activation with particles conjugated with antibodies (Abs) to human CD3, CD28 and CD2 molecules increased the proportion of cells expressing G-CSF receptor (G-CSFR, CD114) in all T cell subpopulations studied (CD45RA+/CD197+ naive T cells, CD45RA−/CD197+ central memory T cells, CD45RA−/CD197− effector memory T cells and CD45RA+/CD197− terminally differentiated effector T cells). Upon T-cell activation in vitro, G-CSF (10.0 ng/ml) significantly and specifically enhanced the proportion of CD114+ T cells in central memory CD4+ T cell compartment. A dilution series of G-CSF (range, 0.1–10.0 ng/ml) was tested, with no effect on the expression of CD25 (interleukin-2 receptor α-chain) on activated T cells. Meanwhile, G-CSF treatment enhanced the proportion of CD38+ T cells in CD4+ naive T cell, effector memory T cell and terminally differentiated effector T cell subsets, as well as in CD4− central memory T cells and terminally differentiated effector T cells. G-CSF did not affect IL-2 production by T cells; relatively low concentrations of G-CSF down-regulated INF-γ production, while high concentrations of this cytokine up-regulated IL-4 production in activated T cells. The data obtained suggests that G-CSF could play a significant role both in preventing the development of excessive and potentially damaging inflammatory reactivity, and in constraining the expansion of potentially cytodestructive T cells. |
| Databáze: | OpenAIRE |
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