| Autor: |
Igor S. Shiraishi, Renato P. Dorte, Beatriz R Ribeiro, Gabriela C.P.S. Souza, Robert F.H. Dekker, Luciana Furnaleto Maia, Aneli M. Barbosa-Dekker, Dionísio Borsato, Joseph A.M. Evaristo, Geisa P.C. Evaristo, Julliana I. Simionato, Juliana Feijó de Souza Daniel |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Orbital: The Electronic Journal of Chemistry, Vol 13, Iss 5, Pp 404-412 (2021) |
| ISSN: |
1984-6428 |
| Popis: |
The objective of this work was to optimize the culture conditions for laccase production in the presence of Kraft lignin by Fusarium oxysporum JUMAD-053, and to evaluate the biodegradation products of lignin. The fungal isolate that presented highest laccase activity had its production optimized by a statistical factorial design 33 in 15 experimental runs. F. oxysporum presented the highest constitutive laccase titer (5.37 U/mL). Statistical factorial design demonstrated a maximum laccase titer of 9.8 U/mL when assayed against ABTS under the conditions optimized: 1.125% (w/v) yeast extract, 0.5% (w/v) Kraft lignin and 10 days of cultivation. The maximum laccase titer when assayed on DMP was 8.4 U/mL, following the conditions optimized: 1.125% yeast extract, 0.25% Kraft lignin and 7 days of cultivation. The analysis of cultures led to identification of metabolites; two being aromatic: 2,6-dimethoxy benzoic acid and sesamin; also, fumonisin and long-chain fatty acids. As a result of the study, the maximum laccase activities of 9.8 and 8.4 U/mL measured from ABTS and DMP substrates, respectively. The search shows new sources of fungal laccase for obtaining new metabolites of biodegradation from Kraft lignin in culture medium. DOI: http://dx.doi.org/10.17807/orbital.v13i5.1623 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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