The Impact of Limbal Mesenchymal Stromal Cells on Healing of Acute Ocular Surface Wounds Is Improved by Pre-cultivation and Implantation in the Presence of Limbal Epithelial Cells
| Autor: | Nigel L. Barnett, Elham Nili, Neil A. Richardson, Steven Weier, Fiona J. Li, Jennifer Walshe, Rebecca A. Dawson, Damien G. Harkin, Blair McEwan, Cora Lau |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Biomedical Engineering Limbus Corneae Mesenchymal Stem Cell Transplantation 03 medical and health sciences 0302 clinical medicine Eye Injuries Antigen Stroma Cornea Keratin Algerbrush II medicine Animals Humans chemistry.chemical_classification Transplantation Wound Healing ocular surface amniotic membrane business.industry Mesenchymal stem cell Epithelium Corneal limbal mesenchymal stromal cells Epithelial Cells Mesenchymal Stem Cells Cell Biology Original Articles medicine.disease Epithelium eye diseases 030104 developmental biology medicine.anatomical_structure chemistry corneal neovascularization Corneal neovascularization Acute Disease 030221 ophthalmology & optometry Medicine Female Rabbits sense organs business Wound healing |
| Zdroj: | Cell Transplantation, Vol 28 (2019) Cell Transplantation |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | While limbal epithelial cells are used for treating ocular surface wounds, the therapeutic potential of mesenchymal cells cultivated from the limbal stroma (LMSC) is less clear. We have therefore examined the effects of LMSC when applied to acute ocular surface wounds. LMSC derived from male rabbits (RLMSC) were applied to the ocular surface of female rabbits immediately following removal of the corneal and limbal epithelium. Human amniotic membrane (HAM) was used as the vehicle for implanting the RLMSC. The effects of RLMSC were examined when applied alone ( n = 3) and in conjunction with a stratified culture of human limbal epithelial cells (HLE) grown on the opposing surface of the HAM ( n = 3). Outcomes were monitored over 3 months in comparison with animals receiving no treatment ( n = 3) or treatment with HLE alone on HAM ( n = 3). Animals treated with RLMSC ( n = 6) displayed faster re-epithelialization (∼90% versus 70% healing after 12 weeks), with best results being observed when RLMSC were pre-cultivated and implanted in the presence of HLE ( p < 0.01; 90% healing by 7 weeks). While all animals displayed conjunctival cells on the corneal surface (by presence of goblet cells and/or keratin 13 expression) and corneal neovascularization, evidence of corneal epithelial regeneration was observed in animals that received RLMSC in the presence of HLE (by staining for keratin 3 and the absence of goblet cells). Conversely, corneal neovascularization was significantly greater when RLMSC were applied in the absence of HLE ( |
| Databáze: | OpenAIRE |
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