The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains
| Autor: | Rolf Claesson, Dorte Haubek, Jan Oscarsson, C. Höglund Åberg, Anders Johansson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Microbiology (medical)
0301 basic medicine Serotype DNA Bacterial Adolescent Genotype Bacterial Toxins lcsh:QR1-502 Virulence Exotoxins Biology Poster Session Serogroup Aggregatibacter actinomycetemcomitans Ghana Polymerase Chain Reaction Risk Assessment lcsh:Microbiology Microbiology lcsh:Infectious and parasitic diseases 03 medical and health sciences 0302 clinical medicine Periodontal Attachment Loss medicine Journal Article Endocarditis Aggressive periodontitis Humans Dentistry (miscellaneous) lcsh:RC109-216 Longitudinal Studies Child Periodontitis Promoter Regions Genetic Abstract Whole Genome Sequencing Diagnostic marker 030206 dentistry medicine.disease biology.organism_classification Infectious Diseases 030104 developmental biology Genes Bacterial Periodontics Biomarkers |
| Zdroj: | Journal of Oral Microbiology, Vol 9, Iss 0 (2017) Johansson, A, Claesson, R, Åberg, C H, Haubek, D & Oscarsson, J 2017, ' The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains ', Journal of Oral Microbiology, vol. 9, no. Sup1, 1325257 . https://doi.org/10.1080/20002297.2017.1325257 Journal of Oral Microbiology Johansson, A, Claesson, R, Höglund Åberg, C, Haubek, D & Oscarsson, J 2017, ' The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains ', Journal of Periodontal Research, vol. 52, no. 5, pp. 903-912 . https://doi.org/10.1111/jre.12462 |
| ISSN: | 2000-2297 |
| Popis: | BACKGROUND AND OBJECTIVE: Aggregatibacter actinomycetemcomitans is involved in oral and systemic infections, and is associated with, eg aggressive forms of periodontitis and with endocarditis. The cagE gene encodes a ≈39 kDa putative exotoxin expressed by A. actinomycetemcomitans. The level of conservation of cagE, and its possible significance in periodontal disease, has not yet been thoroughly investigated. In the present study, the role of the cagE gene as a diagnostic marker has been investigated.MATERIAL AND METHODS: We have used conventional polymerase chain reaction (PCR), quantitative PCR and whole genome sequencing data to determine the prevalence of cagE in A. actinomycetemcomitans based on analysis of: (i) 249 isolates, collected and cultivated in a Ghanaian longitudinal cohort study; (ii) a serotype b collection of 19 strains; and (iii) the 36 A. actinomycetemcomitans genomes available in the NCBI database.RESULTS: Whereas cagE was absent in the other serotypes, our data support that this gene sequence is linked to a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes of A. actinomycetemcomitans.CONCLUSION: We propose that cagE has the potential to be used as a PCR-based gene marker for the identification of a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes. This finding might be of importance in the risk assessment of the development of periodontal attachment loss in young individuals and hence suggested to be a relevant discovery in future development of new diagnostic tools and/or treatment strategies. |
| Databáze: | OpenAIRE |
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