Disinfection of surfaces contaminated with Clostridioides difficile endospores using NaCl-derived electrochemically activated solution
| Autor: | Ethel Sánchez-Chacón, César Rodríguez, Alejandro Medaglia-Mata, Ricardo Starbird-Pérez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Bleach Sodium Chloride 030501 epidemiology Endospore Sodium dichloroisocyanurate 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents Humans Medicine Spores Bacterial 0303 health sciences Clostridioides difficile 030306 microbiology business.industry Electrochemical Techniques General Medicine Factorial experiment Contamination Spore Disinfection Infectious Diseases chemistry Sodium hypochlorite Cyclic voltammetry 0305 other medical science business Multilocus Sequence Typing Nuclear chemistry |
| Zdroj: | Journal of Hospital Infection. 105:670-677 |
| ISSN: | 0195-6701 |
| DOI: | 10.1016/j.jhin.2020.04.023 |
| Popis: | Summary Background Clostridioides difficile is transmitted through endospores. Most disinfection procedures for these structures deploy high concentrations of chlorine-derived compounds such as sodium hypochlorite (NaOCl) and sodium dichloroisocyanurate (NaDCC). However, these substances are linked to undesirable public health and environmental issues. Aim To compare the efficacy of NaCl-derived electrochemically activated solution (ECAS, 0.18% w/v NaOCl, pH=9.6–10.3), commercial bleach (5000 ppm, 2.83% w/v NaOCl, pH=5.6) and NaDCC (1000 ppm, pH=6.8) to inactivate C. difficile endospores on surfaces using a standard quantitative test (EPA MO-21-03). Findings Ten representative reference and field strains from multi-locus sequence typing Clades 1–5 were assayed (N=10). Irrespective of the phylogenetic background of the strains, ECAS showed comparable or better log reduction values [mean=3.22, 95% confidence interval (CI) 0.40–5.56] than bleach (mean=2.74, 95% CI 0.12–5.50) and NaDCC (mean=2.02, 95% CI 0.10–5.12). Cyclic voltammetry measurements revealed similar electrochemical behaviours and open-circuit potentials for ECAS and NaOCl. Congruently, similar morphologies for spores treated with these two compounds were observed by transmission electron microscopy. A factorial design demonstrated that exposure time, but not activation time, influenced the efficacy of ECAS. Conclusions ECAS and NaOC were found to have functional equivalence and may have a common mechanism of action. |
| Databáze: | OpenAIRE |
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