Transcriptional control of human organic anion transporting polypeptide 2B1 gene
| Autor: | Elena Pomari, Lorenzo Colombo, Alessia Nardi, Luisa Dalla Valle, Andrea Celeghin, Cristina Fiore |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Transcription Genetic Endocrinology Diabetes and Metabolism TATA box Molecular Sequence Data Clinical Biochemistry Organic Anion Transporters Biology Exon shuffling Biochemistry Exon Endocrinology Exon trapping Animals Humans Protein Isoforms Coding region Tissue Distribution Promoter Regions Genetic Molecular Biology Gene Base Sequence Promoter Exons Sequence Analysis DNA Cell Biology TATA Box Alternative Splicing Gene Expression Regulation Molecular Medicine Tandem exon duplication Transcription Initiation Site |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 115:146-152 |
| ISSN: | 0960-0760 |
| Popis: | Organic anion transporting polypeptides (OATPs) are a group of transmembrane carriers with a wide spectrum of amphipathic substrates. In particular, OATP2B1 (previously called OATP-B) can transport steroid hormone conjugates and is expressed in organs with steroidogenic activity, such as placenta, brain and skin. In this work, we have analyzed the transcription of the OATP2B1 gene (SLCO2B1) in 14 different human tissues by means of 5′-RACE analysis. Five promoters (only two of which were present in GenBank), associated with distinct first exons, were found to drive OATP2B1 expression, giving rise to transcripts with unique 5′-untranslated termini. Exon 1b is widely expressed and was found here in 10 tissues. It is partially coding, while the other four different first exons are untranslated. All exons are spliced to a common exon 2 that contains a putative ATG in frame with the following coding region. Sequence analysis of the 5′-flanking region of each first exon revealed a lack of TATA box, thus accounting for the use of multiple transcriptional start sites in nearly all first exons. |
| Databáze: | OpenAIRE |
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