Evaluation of emodepside in laboratory models of human intestinal nematode and schistosome infections

Autor: Cécile Häberli, Ivan Scandale, Anna Neodo, Valérian Pasche, Tanja Karpstein, Jennifer Keiser
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Nematoda
Necator americanus
Nematodes
Drug repurposing
Administration
Oral
Trichuris muris
Feces
Mice
0302 clinical medicine
Cricetinae
Depsipeptides
Schistosomatidae
Anthelmintics
biology
Schistosoma spp
Schistosoma mansoni
Trichuris spp
3. Good health
Trichuris
Infectious Diseases
Female
Strongyloides ratti
medicine.drug
030231 tropical medicine
Trematodes
Microbiology
lcsh:Infectious and parasitic diseases
Hookworm Infections
03 medical and health sciences
parasitic diseases
medicine
Animals
Humans
Helminths
lcsh:RC109-216
Soil-transmitted helminthiases (STH)
Emodepside
Ancylostoma ceylanicum
Mesocricetus
Research
Drug Repositioning
biology.organism_classification
Mice
Inbred C57BL
Disease Models
Animal
030104 developmental biology
Hookworms
Parasitology
Heligmosomoides polygyrus
Zdroj: Parasites & Vectors, Vol 12, Iss 1, Pp 1-10 (2019)
Parasites & Vectors
ISSN: 1756-3305
DOI: 10.1186/s13071-019-3476-x
Popis: Background Helminthiases are very prevalent worldwide, yet their treatment and control rely on a handful of drugs. Emodepside, a marketed broad-spectrum veterinary anthelminthic with a unique mechanism of action, undergoing development for onchocerciasis is an interesting anthelmintic drug candidate. We tested the in vitro and in vivo activity of emodepside on nematode species that serve as models for human soil-transmitted helminth infection as well as on schistosomes. Methods In vitro viability assays were performed over a time course of 72 hours for Trichuris muris, Necator americanus, Ancylostoma ceylanicum, Heligmosomoides polygyrus, Strongyloides ratti, Schistosoma mansoni and Schistosoma haematobium. The drug effect was determined by the survival rate for the larvae and by phenotypical scores for the adult worms. Additionally, mice infected with T. muris and hamsters harboring hookworm infection (N. americanus or A. ceylanicum) were administered orally with emodepside at doses ranging from 1.25 to 75 mg/kg. Expelled worms in the feces were counted until 3 days post-drug intake and worms residing in the intestines were collected and counted after dissection. Results After 24 hours, emodepside was very active in vitro against both larval and adult stages of the nematodes T. muris, A. ceylanicum, N. americanus, H. polygyrus and S. ratti (IC50
Databáze: OpenAIRE
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