State‐of‐the‐art microscopy to understand islets of Langerhans: what to expect next?

Autor: Pascal de Boer, Ben N G Giepmans
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Computer science
Immunology
Reviews
large‐scale electron microscopy
multimodal imaging
Microscopic description
CA2+ DYNAMICS
model systems
03 medical and health sciences
Islets of Langerhans
AGE
0302 clinical medicine
data management and analysis
Special Feature Review
Insulin-Secreting Cells
intravital microscopy
ABLATION
Immunology and Allergy
Animals
large‐
Electron microscopic
Pancreas
ELECTRON-MICROSCOPY
geography
Microscopy
geography.geographical_feature_category
ZEBRAFISH
IN-SITU
Cellular imaging
ORGANELLES
Cell Biology
Islet
scale electron microscopy
Functional imaging
biobank
030104 developmental biology
Diabetes Mellitus
Type 1
RESOLUTION
TISSUE
SPECIAL FEATURE to celebrate 100 years since the discovery of insulin
PANCREATIC BETA-CELLS
Fish
Spatiotemporal resolution
Beta cell
Neuroscience
030215 immunology
Zdroj: Immunology and Cell Biology
ISSN: 1440-1711
0818-9641
Popis: The discovery of Langerhans and microscopic description of islets in the pancreas were crucial steps in the discovery of insulin. Over the past 150 years, many discoveries in islet biology and type 1 diabetes have been made using powerful microscopic techniques. In the past decade, combination of new probes, animal and tissue models, application of new biosensors and automation of light and electron microscopic methods and other (sub)cellular imaging modalities have proven their potential in understanding the beta cell under (patho)physiological conditions. The imaging evolution, from fluorescent jellyfish to real‐time intravital functional imaging, the revolution in automation and data handling and the increased resolving power of analytical imaging techniques are now converging. Here, we review innovative approaches that address islet biology from new angles by studying cells and molecules at high spatiotemporal resolution and in live models. Broad implementation of these cellular imaging techniques will shed new light on cause/consequence of (mal)function in islets of Langerhans in the years to come.
Innovative microscopic approaches that allow to address islet biology from new angles by studying cells and molecules at high spatiotemporal resolution and in live models are reviewed in this article. Broad implementation of these cellular imaging techniques will shed new light into the cause/consequence of (mal)function of islet of Langerhans in the years to come.
Databáze: OpenAIRE
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